色谱

色谱 ›› 2013, Vol. 31 ›› Issue (4): 386-391.DOI: 10.3724/SP.J.1123.2012.12008

• 研究论文 • 上一篇    下一篇

纳升电喷雾-离子迁移谱及其与高效液相色谱的联用

陈创1,2,王卫国1,梁茜茜1,2,周庆华1,2,彭丽英1,2,温萌1,2,鞠帮玉1,赵琨1,刘军3,4,李海洋1*   

  1. 1. 中国科学院大连化学物理研究所, 辽宁 大连 116023;2. 中国科学院大学, 北京 100049;
    3. 南京财经大学, 江苏 南京 210000;4. 江苏省质量安全工程研究院, 江苏 南京 210000
  • 收稿日期:2012-12-03 出版日期:2013-04-22 发布日期:2013-04-24
  • 通讯作者: Tel:(0411)84379510,E-mail:hli@dicp.ac.cn

Nano-electrospray ionization-ion mobility spectrometry and its combination with high performance liquid chromatography

CHEN Chuang1,2, WANG Weiguo1, LIANG Xixi1,2, ZHOU Qinghua1,2, PENG Liying1,2, WEN Meng1,2, JU Bangyu1, ZHAO Kun1, LIU Jun3,4, LI Haiyang1*   

  1. 1. Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China; 2. University of Chinese Academy of Sciences, Beijing 100049, China; 3. Nanjing University of Finance and Economics, Nanjing 210000, China; 4. Jiangsu Province Institute of Quality & Safety Engineering, Nanjing 210000, China
  • Received:2012-12-03 Online:2013-04-22 Published:2013-04-24

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摘要： 搭建了一套纳升级电喷雾-离子源离子迁移谱仪。首先,分别对尾吹气流速、溶剂流速等影响仪器去溶剂化效果的参数进行了研究和优化。在此基础上,用一系列胺类化合物对该仪器的去溶剂化效果、分辨能力以及灵敏度进行了表征。实验结果表明,该仪器能够对电喷雾离子液滴实现完全去溶剂化；三辛胺的检出限可以达到10 μg/L。最后,将该仪器用作高效液相色谱的检测器,在无需衍生化的条件下对胺类混合物样品进行检测。由三乙胺、二乙胺以及丁胺组成的混合样品被成功分离并测定。该系统对三乙胺、二乙胺以及丁胺的线性响应范围均达到近两个数量级。

关键词: 高效液相色谱, 离子迁移谱, 纳升电喷雾离子源, 约化迁移率

Abstract: A nano-electrospray ionization-ion mobility spectrometer (nanoESI-IMS) was built up. Firstly, the effects of the parameters such as drift gas flow rate and solvent flow rate on the desolvation capability were studied and optimized. Then, a series of compounds were used to characterize the nanoESI-IMS system. The results showed that, complete desolvation was achieved for nano-electrospray ion droplets with the nanoESI-IMS apparatus. The limit of detection of this instrument for trioctylamine could reach 10 μg/L. Finally, this instrument was coupled to the high performance liquid chromatography (HPLC) as a detector for amines analysis. A test mixture containing triethylamine, diethylamine and butylamine was successfully separated and determined by the HPLC-nanoESI-IMS system. Linear response ranges of about two orders of magnitude were achieved for triethylamine, diethylamine and butylamine with this system.

Key words: high performance liquid chromatography (HPLC), ion mobility spectrometry, nano-electrospray ionization source, reduced mobility

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