色谱

色谱 ›› 2013, Vol. 31 ›› Issue (10): 1001-1004.DOI: 10.3724/SP.J.1123.2013.03033

• 研究论文 • 上一篇    下一篇

聚类分析辅助中药寡糖电泳分析鉴定中药

郭怀忠1,2, 吴芳1, 张伟泉1, 刘芳黎1   

  1. 1. 河北大学药学院, 河北 保定 071002;
    2. 河北省药物质量分析控制重点实验室, 河北 保定 071002
  • 收稿日期:2013-03-15 修回日期:2013-04-25 出版日期:2013-10-28 发布日期:2013-10-08
  • 通讯作者: 郭怀忠

Identification of traditional Chinese medicines by oligosaccharide electrophoresis analysis assisted with cluster analysis

GUO Huaizhong1,2, WU Fang1, ZHANG Weiquan1, LIU Fangli1   

  1. 1. College of Pharmacy, Hebei University, Baoding 071002, China;
    2. Key Laboratory of Pharmaceutical Quality Control of Hebei Province, Baoding 071002, China
  • Received:2013-03-15 Revised:2013-04-25 Online:2013-10-28 Published:2013-10-08

PDF

280

被引次数

11

摘要：

基于中药多糖结构的复杂性和特征性,针对多糖部分降解后的寡糖片段,建立了一种采用毛细管区带电泳法(CZE)分离分析中药寡糖,并利用其特征性电泳谱图信息,结合聚类分析(CA)进行中药鉴定的方法。该方法以1-苯基-3-甲基-5-吡唑啉酮(PMP)为寡糖柱前衍生化试剂,对3个科属的6种中药如黄精、玉竹等同时进行鉴定。采用的电泳条件:未涂层熔融石英毛细管柱(49 cm(有效长度40 cm)×50 μm),以50 mmol/L磷酸盐缓冲液(pH 2.5)为运行缓冲液,检测波长为245 nm,运行电压为15 kV,虹吸进样10 cm×4 s,柱温为室温。结果表明聚类分析辅助中药寡糖电泳分析法可有效用于3个科属6种中药的鉴定。本方法结果可靠,重现性好,可以作为中药鉴定的一种有效手段。

关键词: 多糖, 寡糖, 鉴定, 聚类分析, 毛细管区带电泳, 中药

Abstract:

The structure of polysaccharide in traditional Chinese medicine (TCM) is complex and characteristic. A method of oligosaccharide electrophoresis analysis assisted with cluster analysis (CA) was established to simultaneously identify TCMs. Six TCMs from three families were selected as experimental subjects and their polysaccharides were extracted. The obtained oligosaccharides via incompletely degraded TCM polysaccharides were derivatized using 1-phenyl-3-methyl-5-pyrazolone (PMP). The PMP-oligosaccharide derivatives were separated and analyzed by capillary zone electrophoresis (CZE). The six TCMs were identified by the featured information of oligosaccharide electropherograms with CA. The electrophoresis conditions were as follows: uncoated fused silica capillary column (49 cm/40 cm (total length/effective length)×50 μm); running buffer solution, 50 mmol/L phosphate buffer solution (pH 2.5); detection wavelength, 245 nm; operating voltage, 15 kV; hydrodynamic pressure injection, 10 cm×4 s. The results showed that the six TCMs from three families were effectively identified by the method of TCM oligosaccharide electropherograms combined with CA. This method is a promising tool to identify TCM with good reliability and reproducibility.

Key words: capillary zone electrophoresis (CZE), cluster analysis (CA), identification, oligosaccharide, polysaccharide, traditional Chinese medicine (TCM)

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