基于稳定同位素标记的蛋白质组学定量方法研究进展

doi:10.3724/SP.J.1123.2013.05007

色谱 ›› 2013, Vol. 31 ›› Issue (6): 496-502.DOI: 10.3724/SP.J.1123.2013.05007

• 特别策划：定量蛋白质组学专栏 • 上一篇下一篇

基于稳定同位素标记的蛋白质组学定量方法研究进展

周愿^1,2, 单亦初¹, 张丽华¹, 张玉奎¹

1. 中国科学院大连化学物理研究所, 中国科学院分离分析化学重点实验室, 国家色谱研究分析中心, 辽宁大连 116023;
2. 中国科学院大学, 北京 100039

收稿日期:2013-05-02 出版日期:2013-06-28 发布日期:2013-06-06
通讯作者: 张丽华
基金资助:
国家重大科学计划"蛋白质定量新方法及相关技术研究"项目(2012CB910604).

Progress in stable isotope labeled quantitative proteomics methods

ZHOU Yuan^1,2, SHAN Yichu¹, ZHANG Lihua¹, ZHANG Yukui¹

1. Key Laboratory of Separation Sciences for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences; National Chromatographic Research and Analysis Center, Dalian 116023, China;
2. University of Chinese Academy of Sciences, Beijing 100039, China

Received:2013-05-02 Online:2013-06-28 Published:2013-06-06
Contact: 10.3724/SP.J.1123.2013.05007

摘要/Abstract

摘要：

定量蛋白质组学已经成为后基因时代的重要研究方向之一。目前该领域的研究主要采用无标记定量方法和稳定同位素标记定量法。其中,基于稳定同位素标记的蛋白质组定量方法发展非常迅速,已为生命科学研究提供了重要的技术支撑。本文分析了基于稳定同位素标记的蛋白质组学定量方法,包括相对定量方法和绝对定量方法,并对其发展进行了展望。

关键词: 定量蛋白质组学, 绝对定量, 稳定同位素标记, 相对定量

Abstract:

Quantitative proteomics is an important research field in post-genomics era. There are two strategies for proteome quantification: label-free methods and stable isotope labeling methods which have become the most important strategy for quantitative proteomics at present. In the past few years, a number of quantitative methods have been developed, which support the fast development in biology research. In this work, we discuss the progress in the stable isotope labeling methods for quantitative proteomics including relative and absolute quantitative proteomics, and then give our opinions on the outlook of proteome quantification methods.

Key words: absolute quantitative, quantitative proteomics, relative quantitative, stable isotope labeling

中图分类号:

O658

周愿, 单亦初, 张丽华, 张玉奎. 基于稳定同位素标记的蛋白质组学定量方法研究进展[J]. 色谱, 2013, 31(6): 496-502.

ZHOU Yuan, SHAN Yichu, ZHANG Lihua, ZHANG Yukui. Progress in stable isotope labeled quantitative proteomics methods[J]. Chinese Journal of Chromatography, 2013, 31(6): 496-502.

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基于稳定同位素标记的蛋白质组学定量方法研究进展

Progress in stable isotope labeled quantitative proteomics methods

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