色谱

色谱 ›› 2014, Vol. 32 ›› Issue (4): 438-442.DOI: 10.3724/SP.J.1123.2013.11020

• 庆祝《色谱》创刊三十周年暨卢佩章院士九十华诞专刊-研究论文 • 上一篇    

食品中柠檬黄铝色淀和日落黄铝色淀的毛细管区带电泳分析

张一丁1, 常翠兰1, 郭启雷2, 曹红2, 白玉1, 刘虎威1   

  1. 1. 北京大学化学与分子工程学院分析化学研究所, 北京 100871;
    2. 北京市海淀区产品质量监督检验所, 北京 100094
  • 收稿日期:2013-11-06 修回日期:2013-12-31 出版日期:2014-04-08 发布日期:2014-03-28
  • 通讯作者: 白玉
  • 基金资助:

    北京市科技计划项目（Z111100056811006）.

Analysis of tartrazine aluminum lake and sunset yellow aluminum lake in foods by capillary zone electrophoresis

ZHANG Yiding1, CHANG Cuilan1, GUO Qilei2, CAO Hong2, BAI Yu1, LIU Huwei1   

  1. 1. Institute of Analytical Chemistry, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China;
    2. Haidian District Institute of Products Quality Supervision and Inspection, Beijing 100094, China
  • Received:2013-11-06 Revised:2013-12-31 Online:2014-04-08 Published:2014-03-28

PDF

399

被引次数

7

摘要：

发展了一种新的采用毛细管区带电泳分析柠檬黄铝色淀和日落黄铝色淀的方法。通过前处理步骤成功实现了铝色淀中铝基质与色素的分离。利用石英毛细管柱（48.50 cm（有效长度40.00 cm）×50 μm），分别针对柠檬黄铝色淀和日落黄铝色淀进行了电泳条件的优化，并得到最优分离结果。所建立的定量分析方法的检出限对于柠檬黄铝色淀和日落黄铝色淀分别达0.26 mg/L和0.27 mg/L，线性范围分别为0.53~1.3×102mg/L和0.54~1.4×102mg/L，两种被分析物的测定重复性（RSD，n=6）分别为4.3%和5.7%，日间重复性（RSD，n=6）分别为5.6%和6.0%。经过更深入研究后，该方法可以发展为食品中相应色淀的检测方法。

关键词: 毛细管区带电泳, 柠檬黄, 日落黄, 色淀, 食品

Abstract:

A novel analytical method for tartrazine aluminum lake and sunset yellow aluminum lake using capillary zone electrophoresis (CZE) was studied. The pigments contained in the color lakes were successfully separated from the aluminum matrix in the pre-treatment process, which included the following steps: dissolve the color lakes in 0.1 mol/L H2SO4, adjust the pH of the solution to 5.0, then mix it with the solution of EDTA·52Na and heat it in a water bath, then use polyamide powder as the stationary phase of solid phase extraction to separate the pigments from the solution, and finally elute the pigments with 0.1 mol/L NaOH. The CZE conditions systematically optimized for tartrazine aluminum lake were: 48.50 cm of a fused silica capillary with 40.00 cm effective length and 50 μm i. d., the temperature controlled at 20.0 ℃, 29.0 kV applied, HPO42--PO43- (0.015 mol/L, pH 11.45) solution as running buffer, detection at 263 nm. The conditions for sunset yellow aluminum lake were: the same capillary and temperature, 25.0 kV applied, HPO42--PO43- (0.025 mol/L, pH 11.45) solution as running buffer, detection at 240 nm. The limits of detection were 0.26 mg/L and 0.27 mg/L, and the linear ranges were 0.53-1.3×102 mg/L and 0.54-1.4×102 mg/L for tartrazine aluminum lake and sunset yellow aluminum lake, respectively. The RSDs were 4.3% and 5.7% (run to run, n=6), 5.6% and 6.0% (day to day, n=6) for tartrazine aluminum lake and sunset yellow aluminum lake, respectively. Further developments for this method could make it a routinely used method analyzing color lakes in foods.

Key words: capillary zone electrophoresis (CZE), color lakes, foods, sunset yellow, tartrazine

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