色谱

色谱 ›› 2014, Vol. 32 ›› Issue (6): 616-622.DOI: 10.3724/SP.J.1123.2014.02010

• 研究论文 • 上一篇    下一篇

高效液相色谱法同时测定食品接触材料中抗氧化剂和紫外吸收剂的迁移量

李成发1, 李英1, 陈枝楠2, 梁烽1, 陈旭辉1, 吴绍精2, 李泳涛1, 孙小颖1   

  1. 1. 深圳出入境检验检疫局, 广东 深圳 518067;
    2. 深圳市检验检疫科学研究院, 广东 深圳 518067
  • 收稿日期:2014-02-17 修回日期:2014-04-09 出版日期:2014-06-08 发布日期:2014-05-22
  • 通讯作者: 李成发
  • 基金资助:

    科技部科技支撑计划项目（2011BAK10B05-03）.

Simultaneous determination of migration amounts of antioxidants and ultraviolet absorbents by high performance liquid chromatography in food contact materials

LI Chengfa1, LI Ying1, CHEN Zhinan2, LIANG Feng1, CHEN Xuhui1, WU Shaojing2, LI Yongtao1, SUN Xiaoying1   

  1. 1. Shenzhen Entry-Exit Inspection and Quarantine Bureau, Shenzhen 518067, China;
    2. Shenzhen Academy of Inspection and Quarantine, Shenzhen 518067, China
  • Received:2014-02-17 Revised:2014-04-09 Online:2014-06-08 Published:2014-05-22

PDF

359

被引次数

37

摘要：

采用高效液相色谱技术，建立了食品接触材料中多种抗氧化剂和紫外吸收剂迁移水平的检测方法。该方法测定的23种目标化合物具有较好的线性关系，相关系数（r2）≥ 0.9998，检出限和定量限分别在0.01到0.22 mg/L之间和0.03到0.85 mg/L之间。依据欧盟指令（EU）No. 10/2011，考察了5种食品模拟物30 g/L乙酸、10%（v/v）乙醇、20%（v/v）乙醇、50%（v/v）乙醇和油类模拟物（异辛烷）中抗氧化剂和紫外吸收剂的迁移量。该方法回收率在92.8%~117.7%之间，相对标准偏差在0.95%~9.72%之间。探讨了不同实验条件对抗氧化剂和紫外吸收剂回收率的影响。结果表明，该方法准确、稳定，完全满足欧盟指令（EU）No 10/2011和GB 9685-2008对食品接触材料及制品中抗氧化剂和紫外吸收剂特定迁移量（SML）的限量要求，并利用该方法测定了30批次食品接触材料中抗氧化剂和紫外吸收剂的迁移水平。

关键词: 高效液相色谱, 抗氧化剂, 迁移, 食品模拟物, 紫外吸收剂

Abstract:

An efficient analytical method for the quantitative determination of migration levels of antioxidants and ultraviolet absorbents in food contact materials by high performance liquid chromatography (HPLC) has been developed. The analytical method showed good linearity with the correlation coefficients (r2) ≥ 0.9998 for all the compounds. The limits of detection were between 0.01 mg/L and 0.22 mg/L and the limits of quantification were in the range of 0.03 to 0.85 mg/L for the 23 analytes. According to the European Union Directive No 10/2011, five food simulants were investigated: 30 g/L acetic acid, 10% (v/v) ethanol, 20% (v/v) ethanol, 50% (v/v) ethanol, and fatty food simulant (isooctane). The recoveries were in the range of 92.8%-117.7%, with the relative standard deviations of 0.95%-9.72%. The effects of different experimental conditions on the recoveries of antioxidants and UV absorbents were studied. The results showed that the method is accurate and stable, and can meet the requirements of European Commission Regulation (EU) No 10/2011 and GB 9685-2008 for the specific migration limits (SML) of the antioxidants and ultraviolet absorbents in real food contact plastic materials and article samples. The method has been applied to determine the migration levels of antioxidants and ultraviolet absorbents in different simulants from the migration tests of 30 batches of food contact material samples.

Key words: antioxidants, food simulants, high performance liquid chromatography (HPLC), migration, ultraviolet absorbents

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