色谱

色谱 ›› 2014, Vol. 32 ›› Issue (7): 762-766.DOI: 10.3724/SP.J.1123.2014.03009

• 技术与应用 • 上一篇    下一篇

高效液相色谱-串联质谱法同时测定辽东楤木不同部位中5种皂苷类成分的含量

马慧1, 宋少江2, 刘菲菲2, 张艳2, 彭缨1   

  1. 1. 沈阳药科大学药学院, 辽宁 沈阳 110016;
    2. 沈阳药科大学中药学院, 辽宁 沈阳 110016
  • 收稿日期:2014-03-10 修回日期:2014-04-16 出版日期:2014-07-08 发布日期:2014-06-25
  • 通讯作者: 彭缨，E-mail：yingpeng1999@163.com.
  • 基金资助:

    国家自然科学青年科学基金项目（81302661）.

Simultaneous determination of five triterpenoid saponins in different parts of Aralia elata by high performance liquid chromatography-tandem mass spectrometry

MA Hui1, SONG Shaojiang2, LIU Feifei2, ZHANG Yan2, PENG Ying1   

  1. 1. School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China;
    2. School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, China
  • Received:2014-03-10 Revised:2014-04-16 Online:2014-07-08 Published:2014-06-25

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摘要：

建立了高效液相色谱-串联质谱法（HPLC-MS/MS）用于测定辽东楤木不同部位中的楤木皂苷Ⅱ、楤木皂苷Ⅳ、楤木皂苷Ⅴ、楤木皂苷Ⅹ和楤木叶皂苷Ⅱ的含量，并比较了楤木不同部位中上述5种皂苷的含量差异。以Alltima C18柱（250 mm×4.6 mm，5 μm）为分析柱，以乙腈和0.1%（体积分数）甲酸水溶液为流动相，梯度洗脱，流速为0.8 mL/min。在电喷雾正离子多反应监测（MRM）模式下进行检测。结果表明该方法中楤木皂苷Ⅱ、楤木皂苷Ⅳ、楤木皂苷Ⅴ、楤木皂苷Ⅹ和楤木叶皂苷Ⅱ分别在0.17~108 μg/L、0.53~329 μg/L、0.77~480 μg/L、0.77~480 μg/L和0.82~510 μg/L范围内线性关系良好，相关系数（r2）均大于0.999，提取回收率为99.0%~100.2%。该方法简单、快速、灵敏，可用于测定辽东楤木药材不同部位中的皂苷类成分的含量。测定结果发现楤木不同部位中上述5种皂苷总量多少的顺序为根皮 >叶 >种子 >芽。

关键词: 辽东楤木, 液相色谱-串联质谱法, 皂苷

Abstract:

A method was developed for the simultaneous determination of congmunoside Ⅱ, congmunoside Ⅳ, congmunoside Ⅴ, congmunoside Ⅹ and congmuyenoside Ⅱ in different parts of Aralia elata by high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). The chromatographic separation was performed on an Alltima C18 analytical column (250 mm×4.6 mm, 5 μm) using the mobile phases of acetonitrile and 0.1% (v/v) formic acid aqueous solution with gradient elution. The separated compounds were detected in multiple reaction monitoring (MRM) mode via positive electrospray ionization (ESI+). The linear ranges of congmunoside Ⅱ, congmunoside Ⅳ, congmunoside Ⅴ, congmunoside Ⅹ and congmuyenoside Ⅱ were 0.17-108 μg/L, 0.53-329 μg/L, 0.77-480 μg/L, 0.77-480 μg/L and 0.82-510 μg/L, respectively. The extraction recoveries varied from 99.0% to 100.2%. The developed method is rapid, accurate and reproducible, and is successfully utilized as a quality control method for Aralia elata. The results indicate that the order of contents of these five saponins in different parts of Aralia elata is root bark >leaves >seeds >buds.

Key words: Aralia elata, high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS), triterpenoid saponins

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