色谱

色谱 ›› 2015, Vol. 33 ›› Issue (3): 314-317.DOI: 10.3724/SP.J.1123.2014.10030

• 技术与应用 • 上一篇    下一篇

超高效聚合物色谱法测定酚化解聚木质素的相对分子质量

崔朋1,2, 方红霞1, 吴强林1, 钱晨1   

  1. 1. 黄山学院应用化学研究所, 安徽 黄山 245041;
    2. 黄山学院分析测试中心, 安徽 黄山 245041
  • 收稿日期:2014-10-31 修回日期:2014-12-02 出版日期:2015-03-08 发布日期:2015-02-12
  • 通讯作者: 方红霞
  • 基金资助:

    安徽省自然科学基金项目(1208085MB33).

Relative molecular mass determination of phenolated depolymerized sodium lignosulfonate by advanced polymer chromatography system

CUI Peng1,2, FANG Hongxia1, WU Qianglin1, QIAN Chen1   

  1. 1. Applied Chemistry Laboratory, Huangshan University, Huangshan 245041, China;
    2. Analysis and Test Center, Huangshan University, Huangshan 245041, China
  • Received:2014-10-31 Revised:2014-12-02 Online:2015-03-08 Published:2015-02-12

PDF

328

被引次数

5

摘要：

超高效聚合物色谱法(APC)是一种新开发的高分辨聚合物表征技术,能在10 min内完成聚合物的高效分辨分离,同时获得聚合物的平均相对分子质量及其分布参数。实验以水溶性热酚化解聚木质素为研究对象,探索了APC测试水性体系高分子相对分子质量及其分布参数的条件。在以弱碱性磷酸盐缓冲液为流动相,采用Waters水溶性ACQUITY APC AQ 450 Å、200 Å、45 Å 3根色谱柱串联,进样量为10 μL,流速为0.5 mL/min的条件下,获得了复杂水相体系木质素磺酸盐解聚产物的平均相对分子质量及其分布参数,且相对标准偏差(RSD)低于1%。结果表明,与传统的凝胶渗透色谱相比,APC方法具有分辨率高、重现性强的优点。本实验为高性能天然高分子的高效分离、结构分析提供了新的方法和途径,同时为木质素的解聚和液化产物调控机理的研究提供了可行的方法。

关键词: 超高效聚合物色谱, 分散指数, 解聚, 木质素磺酸钠, 平均相对分子质量, 水相

Abstract:

Advanced polymer chromatography (APC) is a newly developed high resolution polymer characterization technique. Within 10 min, it can separate polymers with high resolution and obtain the average relative molecular mass distribution parameters of the polymers. The Mr distribution of the depolymerized lignin product was well characterized with low relative standard deviation (RSD less than 1%) by APC system using ACQUITY AQ 450 Å, 200 Å, and 45 Å columns in series, with 10 μL injection volume, and weak alkaline phosphate buffer solution as the aqueous mobile phase at a flow rate of 0.5 mL/min. Compared with traditional gel permeation chromatography (GPC), the APC system had the advantages of high sensitivity and good reproducibility. The technique to determine the average relative molecular mass distribution of depolymerized lignin oligomers in aqueous phase described in this paper will pave the way for new methods to separate and analyze high performance natural materials, and the feasible method for the investigation of the mechanism of depolymerization and the regulation for liquefied lignin would also be established.

Key words: advanced polymer chromatography (APC), aqueous phase, average relative molecular mass, depolymerization, polydispersity index (PDI), sodium lignosulfonate

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