聚(苯乙烯-co-甲基丙烯酸)自组装胶束假固定相电动色谱的特性

doi:10.3724/SP.J.1123.2014.12045

色谱 ›› 2015, Vol. 33 ›› Issue (4): 403-407.DOI: 10.3724/SP.J.1123.2014.12045

聚(苯乙烯-co-甲基丙烯酸)自组装胶束假固定相电动色谱的特性

马新宇¹, 倪鑫炯¹, 卢婕¹, 邢晓平², 曹玉华¹, 曹光群¹

1. 江南大学化学与材料工程学院, 江苏无锡 214122;
2. 盐城工学院化学与生物工程学院, 江苏盐城 224051

收稿日期:2014-12-31 修回日期:2015-01-30 出版日期:2015-04-08 发布日期:2015-03-30
通讯作者: 曹玉华,曹光群
基金资助:
国家自然科学基金项目(21174056,21405133).

Electrokinetic chromatographic properties of amphiphilic copolymer poly (styrene-co-methacrylic acid) self-assembled micelle pseudostationary phase

MA Xinyu¹, NI Xinjiong¹, LU Jie¹, XING Xiaoping², CAO Yuhua¹, CAO Guangqun¹

1. School of Chemical and Material Engineering, Jiangnan University, Wuxi 214122, China;
2. Chemical and Biological Engineering College, Yancheng Institute of Technology, Yancheng 224051, China

Received:2014-12-31 Revised:2015-01-30 Online:2015-04-08 Published:2015-03-30

摘要/Abstract

摘要：

研究了两亲性无规共聚物聚(苯乙烯-co-甲基丙烯酸)(P(St-co-MAA))(单体摩尔比分别为6:4和7:3)自组装胶束的物理化学性质,及其作为假固定相(PSP)的胶束电动色谱性能。测定了聚合物胶束的临界胶束浓度(CMC),对胶束内核微环境的极性、表面电荷密度和流体力学直径等微结构参数进行了表征,对时间窗口、亚甲基选择性等电动色谱参数进行了测定,并与聚(甲基丙烯酸甲酯-co-甲基丙烯酸)(P(MMA-co-MAA))胶束、十二烷基硫酸钠(SDS)胶束体系进行了比较;利用线性溶剂化能关系(LSER)研究了聚合物PSP的选择性差异。结果表明:P(St-co-MAA)体系具有最小的CMC、最宽的时间窗口和最好的亚甲基选择性;LSER表明,疏水作用是决定聚合物PSP选择性的最主要因素,氢键酸度其次,特别是P(St-co-MAA)(单体摩尔比7:3)体系具有最高的作用参数,显示了该PSP具有较高的分离选择性。

关键词: 假固定相, 胶束电动色谱, 两亲性无规共聚物, 线性溶剂化能关系

Abstract:

The amphiphilic copolymer poly (styrene-co-methacrylic acid) (P(St-co-MAA)) with molar ratios of 6:4 and 7:3 self-assembled to form micelles. The polymeric micelles were used as pseudostationary phase (PSP) in micellar electrokinetic chromatography (MEKC). Their physicochemical properties and MEKC performance were investigated as well in the present work. The critical micelle concentration (CMC), polarity, surface charge density and hydrodynamic diameter were used to characterize the solution physicochemical properties, while the methylene group selectivity was evaluated with n-alkylphenone homologous series. The time window and linear solvation energy relationship (LSER) analysis were used to characterize the MEKC retention behavior and the selectivity. All of these were compared with poly (methyl methacrylate-co-methacrylic acid) (P(MMA-co-MAA)) with the molar ratio of 7:3 and sodium dodecyl sulfate (SDS) micellar systems. The results showed that P(St-co-MAA) system had the minimum CMC, the widest time window and the best methylene group selectivity. LSER analysis results showed that the hydrophobic effect was the most important interaction between solutes and PSPs, and the hydrogen-bonding acidity was the second significant factor on selectivity and MEKC retention behavior. P(St-co-MAA) system, especially with the molar ratio of 7:3, had the highest effective parameter in LSER and showed a high separation selectivity of PSP.

Key words: amphiphilic random copolymer, linear solvation energy relationships (LSER), micellar electrokinetic chromatography (MEKC), pseudostationary phase (PSP)

中图分类号:

O658

马新宇, 倪鑫炯, 卢婕, 邢晓平, 曹玉华, 曹光群. 聚(苯乙烯-co-甲基丙烯酸)自组装胶束假固定相电动色谱的特性[J]. 色谱, 2015, 33(4): 403-407.

MA Xinyu, NI Xinjiong, LU Jie, XING Xiaoping, CAO Yuhua, CAO Guangqun. Electrokinetic chromatographic properties of amphiphilic copolymer poly (styrene-co-methacrylic acid) self-assembled micelle pseudostationary phase[J]. Chinese Journal of Chromatography, 2015, 33(4): 403-407.

参考文献

[1] Terabe S, Otsuka K, Ichikawa K, et al. Anal Chem, 1984, 56(1): 111
[2] Liu X Y, Kim J S, Wu J, et al. Macromolecules, 2005, 38(16): 6749
[3] Shi W, Palmer C P. J Sep Sci, 2002, 25(8): 543
[4] Palmer C P, McNair H M. J Microcolumn Sep, 1992, 4(6): 509
[5] Palmer C P, Khaled M Y, McNair H M. HRC-J High Res Chrom, 1992, 15(11): 756
[6] Palmer C P, Terabe S. J Microcolumn Sep, 1996, 8(2): 115
[7] Gao J J, Latep N, Ge Y, et al. J Sep Sci, 2013, 36: 1575
[8] Ni X J, Xing X P, Cao Y H, et al. J Chromatogr A, 2014, 1370: 263
[9] Wang B N, Ni X J, Yu M J, et al. J Chromatogr A, 2012, 1245: 190
[10] Xu X J, Ni X J, Cao Y H, et al. Electrophoresis, 2014, 35: 827
[11] Ahmed H H, Ahlstrom D M, Arslan H, et al. J Chromatogr A, 2012, 1236: 207
[12] Akbay C, Ahmed H H, Arslan H, et al. Talanta, 2012, 99: 441
[13] Lu J, Ni X J, Cao Y H, et al. J Chromatogr A, 2014, 1359: 296
[14] Li H, Ding G S, Yue C Y, et al. Electrophoresis, 2012, 33: 2012
[15] Ahlstrom D M, Hoyos Y M, Arslan H, et al. J Chromatogr A, 2010, 1217: 375
[16] Rosés M, Bosch E. Anal Chim Acta, 1993, 274(1): 147
[17] Abraham M H, Andonian-Haftvan J, Whiting G S, et al. J Chem Soc, Perkin Trans 2, 1994: 1777
[18] Liu Z, Zou H F, Ye M L, et al. J Chromatogr A, 1999, 863: 69
[19] Sykora D, Rídká K, Tesarová E, et al. J Chromatogr A, 2014, 1371: 220
[20] Ulrich N, Mühlenbergc J, Schüürmann G, et al. J Chromatogr A, 2014, 1324: 96
[21] Schuster G, Lindner W. J Chromatogr A, 2013, 1301: 91
[22] Ni X J, Zhuo X L, Xu X J, et al. J Chromatogr A, 2014, 1365: 2195
[23] Phillips J N. Trans Faraday Soc, 1955, 51: 561
[24] Tanaka N, Goodell H, Karger B L. J Chromatogr A, 1978, 158: 233
[25] Potocek B, Chmela E, Maichel B, et al. Anal Chem, 2000, 72(1): 74
[26] Akbay C, Agbaria R A, Warner I M. Electrophoresis, 2005, 26(2): 426
[27] Ahmed H H, Ahlstrom D M, Arslan H, et al. J Chromatogr A, 2012, 1236: 207

聚(苯乙烯-co-甲基丙烯酸)自组装胶束假固定相电动色谱的特性

Electrokinetic chromatographic properties of amphiphilic copolymer poly (styrene-co-methacrylic acid) self-assembled micelle pseudostationary phase

PDF

可视化

被引次数

摘要/Abstract

引用本文

使用本文

扫码分享

参考文献

相关文章 15

编辑推荐

Metrics

[1]	张明瑜, 康经武. 胶束电动色谱柱上酶反应测定低分子量肝素的抗凝血活性[J]. 色谱, 2020, 38(10): 1243-1248.
[2]	高芳芳, 刘军英, 鹿文慧, 李金花, 刘惠涛. 毛细管电泳结合压力辅助电动进样测定水样中4种酚类雌激素[J]. 色谱, 2018, 36(6): 573-577.
[3]	孙建芝, 贺晖, 刘书慧. 分散液液微萃取-反相液液微萃取-扫集-胶束电动色谱法测定红酒中的3种氯酚类物质[J]. 色谱, 2014, 32(3): 256-262.
[4]	胡梦迪1,2, 苏迪1,2, 范晓苏1,2, 于建华2, 徐远金1,2*. 月桂酰基苹果酸酯胶束电动色谱-质谱法同时测定牛黄上清片中5种生物碱[J]. 色谱, 2012, 30(12): 1276-1281.
[5]	王宇飞, 杨甲甲, 蔡元丽, 林夏, 李晖*. 胶束电动色谱-激光诱导荧光检测法测定肌松弛药巴氯芬[J]. 色谱, 2011, 29(12): 1230-1235.
[6]	余美娟, 杭栋, 曹玉华*. 离子液体对胶束电动色谱胶束微结构以及分离效果的影响[J]. 色谱, 2011, 29(02): 131-136.
[7]	米璇,朱若华*. 毛细管胶束电动色谱法分离测定中药半枝莲中的7种有效成分[J]. 色谱, 2010, 28(2): 209-214.
[8]	陈新1, 袁红萍2, 曹玉华1*, 陈秋云1. 阴离子耗尽进样胶束扫集毛细管电动色谱法在线富集测定甘草黄酮类化合物[J]. 色谱, 2010, 28(09): 889-892.
[9]	张琦,叶能胜,谷学新,郝晓丽,刘妮. 固相萃取-胶束电动色谱法测定牛奶中的4种β-内酰胺类抗生素[J]. 色谱, 2008, 26(6): 682-686.
[10]	范恩国,黄晓晶,张锴,白玉华,高如瑜,阎超. 胶束电动色谱法和微柱液相色谱法分析白藜芦醇的比较 [J]. 色谱, 2007, 25(3): 319-321.
[11]	黄锐,穆小静,阴永光,魏为力,陈志涛,夏之宁. 非水胶束电动色谱分离邻苯二甲酸酯类化合物 [J]. 色谱, 2006, 24(6): 597-600.
[12]	张普敦,熊建辉,郑育芳,石先哲,杨青,魏复盛,许国旺. 毛细管电泳法分析维甲酸异构体[J]. 色谱, 2003, 21(6): 577-579.
[13]	宋冠群,董文举,林金明,屈锋. 茶叶指纹谱的毛细管胶束电动色谱法研究[J]. 色谱, 2003, 21(4): 359-362.
[14]	徐小华,张蓉真,盛思梅,陈天豹,李珑,饶平凡. 毛细管胶束电动色谱法测定血管紧张素转化酶的活性[J]. 色谱, 2001, 19(1): 68-70.
[15]	柳常青,张斌,王培荣,方孝林,李红,马亭. 油田水中烷基酚的胶束电动色谱分离[J]. 色谱, 1999, 17(3): 236-239.