核壳结构的萘夫西林磁性分子印迹聚合物微球的制备及性能

doi:10.3724/SP.J.1123.2014.12040

色谱 ›› 2015, Vol. 33 ›› Issue (5): 481-487.DOI: 10.3724/SP.J.1123.2014.12040

• 特别策划：新型分离材料专栏 • 上一篇下一篇

核壳结构的萘夫西林磁性分子印迹聚合物微球的制备及性能

陈朗星¹, 刘雨星¹, 何锡文¹, 张玉奎^1,2

1. 南开大学化学学院, 分析科学研究中心, 天津 300071;
2. 中国科学院大连化学物理研究所, 辽宁大连 116023

收稿日期:2014-12-30 发布日期:2015-04-23
通讯作者: 陈朗星
基金资助:
国家重点基础研究发展规划("973"计划)项目(2012CB910601);国家自然科学基金项目(21275080);高等学校博士学科点专项科研基金项目(20120031110007).

Preparation and characterization of core-shell structural magnetic molecularly imprinted polymers for nafcillin

CHEN Langxing¹, LIU Yuxing¹, HE Xiwen¹, ZHANG Yukui^1,2

1. College of Chemistry, Research Center for Analytical Sciences, Nankai University, Tianjin 300071, China;
2. Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China

Received:2014-12-30 Published:2015-04-23

摘要/Abstract

摘要：

以表面修饰双键的Fe₃O₄@SiO₂纳米颗粒为基体,以萘夫西林(nafcillin)为模板,甲基丙烯酸(MAA)为单体,乙二醇二甲基丙烯酸酯(EGDMA)为交联剂,偶氮二异丁腈(AIBN)为引发剂,采用三步升温聚合法合成了核壳结构的萘夫西林磁性分子印迹聚合物。采用傅里叶变换红外光谱仪(FT-IR)、透射电子显微镜(TEM)、X射线衍射仪(XRD)和振动样品磁强计(VSM)对制备的印迹聚合物微球进行了表征,得到的磁性印迹聚合物微球的粒径在320 nm左右,大小均匀,分散性较好,可以在外加磁场下与溶剂实现快速分离。对磁性印迹和非印迹聚合物进行了吸附性能研究,结果表明该印迹聚合物微球对模板分子具有很高的吸附容量(50.7 mg/g),特异性识别性能良好(印迹因子为2.46),有望应用于实际样品中萘夫西林残留量的富集分析。

关键词: 磁性微球, 分子印迹聚合物, 富集, 萘夫西林

Abstract:

The uniform core-shell nanostructured magnetic molecularly imprinted polymers (MIPs) were synthesized using antibiotic nafcillin as a template. In this protocol, the magnetite nanoparticles (NPs) were synthesized by the solvothermal reaction firstly. Subsequently, the vinyl groups were grated onto silica-modified Fe₃O₄ surface by 3-methacryloyloxypropyltrimethoxysilane via sol-gel method. Finally, the nafcillin-MIPs film was formed on the surface of Fe₃O₄@SiO₂ by the copolymerization of vinyl end group with functional monomer, methacrylic acid, cross-linking agent, ethylene glycol dimethacrylate, the initiator azo-bis-isobutyronitrile and template molecule. The morphological and magnetic characteristics of the MIPs were characterized by transmission electron microscopy, Fourier transform infrared spectroscopy, X-ray diffraction and vibrating sample magnetometer. The obtained spherical magnetic MIPs with diameters of about 320 nm had good monodispersity. The static binding experiment was carried out to evaluate the properties of magnetic MIPs and non imprinted polymers (NIPs). The results demonstrated that the magnetic MIPs had high adsorption capacity to template and good selectivity. The imprinting factor and the maximum adsorption capacity of Fe₃O₄@MIPs to nafcillin were 2.46 and 50.7 mg/g, respectively. It is expected that the prepared magnetic MIPs could be used for the enrichment of nafcillin in complex samples.

Key words: enrichment, magnetic microspheres, molecularly imprinted polymers (MIPs), nafcillin

中图分类号:

O658

陈朗星, 刘雨星, 何锡文, 张玉奎. 核壳结构的萘夫西林磁性分子印迹聚合物微球的制备及性能[J]. 色谱, 2015, 33(5): 481-487.

CHEN Langxing, LIU Yuxing, HE Xiwen, ZHANG Yukui. Preparation and characterization of core-shell structural magnetic molecularly imprinted polymers for nafcillin[J]. Chinese Journal of Chromatography, 2015, 33(5): 481-487.

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核壳结构的萘夫西林磁性分子印迹聚合物微球的制备及性能

Preparation and characterization of core-shell structural magnetic molecularly imprinted polymers for nafcillin

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