色谱

色谱 ›› 2015, Vol. 33 ›› Issue (6): 571-576.DOI: 10.3724/SP.J.1123.2015.03019

• 研究论文 • 上一篇    下一篇

固相萃取-超高效液相色谱-串联质谱法同时测定食品接触塑料制品中10种苯并三唑类紫外吸收剂

勾新磊1, 赵新颖1, 池海涛1, 高峡1,2, 周明强1, 刘伟丽1,2   

  1. 1. 北京市理化分析测试中心, 有机材料检测技术与质量评价北京市重点实验室, 北京 100089;
    2. 北京市食品安全分析测试工程技术研究中心, 北京 100089
  • 收稿日期:2015-03-16 出版日期:2015-06-08 发布日期:2015-05-26
  • 通讯作者: 刘伟丽
  • 基金资助:

    北京市自然科学基金项目(2122018);北京市科学技术研究院萌芽计划项目(2015-02).

Simultaneous determination of ten benzotriazole ultraviolet stabilizers in food contact plastic materials by solid phase extraction and ultra performance liquid chromatography with tandem mass spectrometry

GOU Xinlei1, ZHAO Xinying1, CHI Haitao1, GAO Xia1,2, ZHOU Mingqiang1, LIU Weili1,2   

  1. 1. Beijing Key Laboratory of Detection Technology and Quality Evaluation of Organic Material, Beijing Center for Physical and Chemical Analysis, Beijing 100089, China;
    2. Beijing Engineering Research Center of Food Safety Analysis, Beijing 10089, China
  • Received:2015-03-16 Online:2015-06-08 Published:2015-05-26

PDF

235

被引次数

20

摘要：

建立了固相萃取-超高效液相色谱-串联质谱(SPE-UPLC-MS/MS)同时测定食品接触塑料制品中10种苯并三唑类(BZTs)紫外吸收剂的方法。食品接触塑料制品使用甲醇-二氯甲烷混合溶剂超声提取,C18固相萃取柱净化后用Waters ACQUITY UPLC BEH C18色谱柱(100 mm×2.1 mm, 1.7 μm)分离,甲醇和0.1%(v/v)甲酸水溶液为流动相进行梯度洗脱,多反应监测(MRM)模式检测,外标法定量分析。结果表明,10种BZTs在线性范围内线性关系良好,线性相关系数(r2)均大于0.996;检出限为0.6~1.6 μg/kg; 3个水平下的加标回收率为75.2%~85.3%,相对标准偏差为1.0%~5.7%。该方法准确、简便、快速、检出限低,可用于食品接触塑料制品中10种BZTs的同时检测。

关键词: 苯并三唑类化合物, 超高效液相色谱-串联质谱, 食品接触塑料制品, 紫外吸收剂

Abstract:

A sensitive method was developed for the simultaneous determination of ten benzotriazole ultraviolet stabilizers in food contact plastic materials by ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). The sample was extracted by methanol-dichloromethane, and purified by a C18 solid-phase extraction (SPE) column. The separation was performed by using water containing 0.1% (v/v) formic acid and methanol as the mobile phases with gradient elution at a flow rate of 0.3 mL/min. The electrospray ionization (ESI) source in positive ion mode was used for the analysis of the ten benzotriazole ultraviolet stabilizers in multiple reaction monitoring (MRM) mode. The results showed that the standard curves were obtained with good correlation coefficients (r2>0.996) in their linear concentration ranges. The limits of detection (LODs, S/N=3) for the ten benzotriazole ultraviolet stabilizers were in the range of 0.6-1.6 μg/kg. The mean recoveries for the ten benzotriazole ultraviolet stabilizers at three spiked levels (low, medium and high) were 75.2%-85.3% with relative standard deviations of 1.0%-5.7%. Ten kinds of food contact plastic materials were tested, and 2,2'-methylenebis(6-(benzotriazol-2-yl)-4-tert-octylphenol) (UV-360) was found in a sample of polyethylene (PE) material. The method is accurate, simple, rapid and feasible for the simultaneous determination of benzotriazole ultraviolet stabilizers in food plastic materials.

Key words: benzotriazoles (BZTs), food contact plastic materials, ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS), ultraviolet stabilizers

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