色谱

色谱 ›› 2015, Vol. 33 ›› Issue (6): 577-582.DOI: 10.3724/SP.J.1123.2015.01020

• 研究论文 • 上一篇    下一篇

超高效液相色谱-串联质谱法测定甜樱桃果皮中花青苷类物质

魏海蓉1, 易锡斌2, 谭钺1, 宗晓娟1, 王甲威1, 徐丽1, 刘庆忠1   

  1. 1. 山东省果树研究所, 山东省果树生物技术育种重点实验室, 山东 泰安 271000;
    2. 山东农业大学园艺科学与工程学院, 山东 泰安 271018
  • 收稿日期:2015-01-16 出版日期:2015-06-08 发布日期:2015-05-26
  • 通讯作者: 刘庆忠
  • 基金资助:

    "十二五"国家科技支撑计划项目子课题(2013BAD02B03-3-2);山东省现代农业产业技术体系水果创新团队专项基金项目(SDAIT-03-022-04);山东省农业科学院青年科研基金项目(2014QNM33).

Determination of anthocyanins in the peel of sweet cherry by ultra performance liquid chromatography-tandem mass spectrometry

WEI Hairong1, YI Xibin2, TAN Yue1, ZONG Xiaojuan1, WANG Jiawei1, XU Li1, LIU Qingzhong1   

  1. 1. Shandong Key Laboratory for Fruit Biotechnology Breeding, Shandong Institute of Pomology, Taian 271000, China;
    2. College of Horticulture Science and Engineering, Shandong Agricultural University, Taian 271018, China
  • Received:2015-01-16 Online:2015-06-08 Published:2015-05-26

PDF

234

被引次数

7

摘要：

建立了甜樱桃果皮中7种花青苷类物质的超高效液相色谱-串联质谱(UPLC-MS/MS)定性和定量检测方法。样品经0.1%(v/v)盐酸甲醇提取液提取,AB-8型大孔吸附树脂净化,在电喷雾离子源正离子(ESI+)多反应监测(MRM)模式下对目标物质进行定性和定量分析。结果表明:7种目标化合物的定量限为0.26~1.42 μg/kg;在0~100 μg/L范围内呈现很好的线性关系,相关系数(r2)为0.9964~0.9993;方法的加标回收率为97.2%~105.4%,相对标准偏差(RSD)为1.9%~5.8%。采用该方法对甜樱桃红色品种"美早"和黄色品种"13-33"成熟果实样品进行了分析,发现主要花青苷类物质的成分和含量存在显著性差异。该方法操作简单,灵敏度高,重复性好,花青苷类物质的分析覆盖面广,可用于甜樱桃中花青苷类成分快速鉴定及含量测定。

关键词: AB-8大孔吸附树脂, 超高效液相色谱-串联质谱, 花青苷, 甜樱桃

Abstract:

A method for the determination of seven anthocyanins in the peel of sweet cherry was developed by ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). The sample was extracted by methanol containing 0.1%(v/v) hydrochloric acid, and then purified by AB-8 macroporous resins. The separation was carried out on a Phenomenex Kinetex column (100 mm×4.6 mm, 2.6 μm) with mobile phase of 0.1%(v/v) formic acid aqueous solution containing 5 mmol/L ammonium formate and methanol. The sample solution was detected by UPLC-MS/MS with ESI under positive ion and multi reaction monitoring (MRM) modes. The results showed that the limits of quantification (LOQs) for the seven target compounds were 0.26-1.42 μg/kg. The seven anthocyanin standards showed a good linearity in the range of 0-100 μg/L with the correlation coefficients (r2) of 0.9964-0.9993. The average recoveries of the seven anthocyanins were 97.2%-105.4%, and the relative standard deviations (RSDs) were 1.9%-5.8%. The mature fruit samples of sweet cherry red variety "Tieton" and the yellow variety "13-33" were analyzed by this method. The results showed that the anthocyanin composition and contents were significantly different between the two varieties. This method can be used for rapid identification and the determination of anthocyanin components in sweet cherry fruits due to its simple operation, high sensitivity, good reproducibility and covering a wide range of anthocyanins.

Key words: AB-8 macroporous resins, anthocyanins, sweet cherry, ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS)

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