关键词: 超高效液相色谱, 多酚化合物, 高分辨质谱, 葡萄酒, 线性离子阱/静电场轨道阱质谱

Abstract:

Wine has been described previously as a rich source of polyphenols. However, an accurate screening of its complete phenolic profile is still lacking. In the present work, the analysis of 38 polyphenols in wine using ultra high performance liquid chromatography-linear ion trap/orbitrap high resolution mass spectrometry (UPLC-LTQ/Orbitrap MS) was explored. Wines were directly detected. The sample was loaded onto a Thermo Hypersil Gold C18 column (100 mm×2.1 mm, 1.9 μm) using a gradient elution of acetonitrile/water containing 0.1% (v/v) formic acid for the separation. UPLC-LTQ/Orbitrap mass spectrometer acquired full scan MS date for quantification, and data dependent MS² product ion spectra for identification and/or confirmation. The regression coefficients (R²) for the calibration curves (two orders of magnitude up to the lowest calibration level) in the study were ≥0.99. The limits of detection for the 38 compounds were 0.002-0.50 mg/kg. The average recoveries at three spiked levels were in the range of 90%-102% with the relative standard deviations (RSDs) of 0.51%-2.56%. Mass errors were always ≤ 5 ppm. This procedure was then successfully applied to the analysis of the polyphenols in wines.

Key words: high resolution mass spectrometry (HRMS), linear ion trap/orbitrap mass spectrometry (LTQ/Orbitrap MS), polyphenols, ultra high performance liquid chromatography (UPLC), wine