色谱

色谱 ›› 2018, Vol. 36 ›› Issue (4): 334-338.DOI: 10.3724/SP.J.1123.2017.10007

• 研究论文 • 上一篇    下一篇

基于天冬氨酸的固定化金属离子亲和色谱材料用于磷酸化肽选择性富集

申惠莲1, 卡哈尔·阿里木2   

  1. 1. 辽宁民族师范高等专科学校, 辽宁 沈阳 110032;
    2. 新疆农业职业技术学院, 新疆 昌吉 831100
  • 收稿日期:2017-10-20 出版日期:2018-04-08 发布日期:2014-02-26
  • 通讯作者: 申惠莲,Tel:(024)86622204,E-mail:1055824094@qq.com

Selective enrichment of phosphopeptides with aspartic acid based immobilized metal ion affinity chromatography materials

SHEN Huilian1, ALIMU Kahaer2   

  1. 1. Liaoning National Normal College, Shenyang 110032, China;
    2. Xinjiang Agricultural Vocational Technical College, Changji 831100, China
  • Received:2017-10-20 Online:2018-04-08 Published:2014-02-26

PDF

65

摘要：

采用点击化学的方法将自然界中的天冬氨酸（aspartic acid）键合到硅球上（命名为Click Asp），并将Fe3+配位到Click Asp上，合成固定金属离子亲和色谱（IMAC）材料（Fe3+-Click Asp）；采用红外光谱、X射线电子能谱和扫描电镜等表征证明Fe3+-Click Asp成功合成。将此IMAC材料用于蛋白质酶解液和牛奶中的磷酸化肽的富集，实现了磷酸化肽的高选择性富集。本研究为磷酸化蛋白质组学提供了新材料和新方法。

关键词: 富集, 固定化金属离子亲和色谱, 固相萃取, 磷酸化肽, 天冬氨酸

Abstract:

A natural amino acid, aspartic acid, was modified to bind to silica microspheres with a click chemistry method (-Click Asp).The modified Click Asp microspheres were further modified by incorporating Fe3+ ions, resulting in Fe3+ and Click Asp based immobilized ion affinity chromatography (IMAC)(Fe3+-Click Asp).The prepared materials were characterized using Fourier transform infrared spectrometry, X-ray photoelectron spectrometry, and scanning electron microscopy, and it was confirmed that Fe3+-Click Asp was successfully prepared.Subsequently, the Fe3+-Click Asp microspheres were used to enrich phosphopeptides from tryptic digests of proteins and milk.It was found that the phosphopeptides could be selectively enriched with this material.This study describes a novel material and enrichment method for phosphoproteomics.

Key words: aspartic acid, enrichment, immobilized metal ion affinity chromatography (IMAC), phosphopeptide, solid phase extraction (SPE)

中图分类号: