超高效液相色谱-三重四极杆质谱法同时检测人尿中12种单羟基多环芳烃代谢物

doi:10.3724/SP.J.1123.2017.12029

色谱 ›› 2018, Vol. 36 ›› Issue (5): 487-492.DOI: 10.3724/SP.J.1123.2017.12029

超高效液相色谱-三重四极杆质谱法同时检测人尿中12种单羟基多环芳烃代谢物

付慧, 胡小键, 陈曦, 林少彬

中国疾病预防控制中心环境与健康相关产品安全所, 北京 100021

收稿日期:2017-12-28 出版日期:2018-05-08 发布日期:2014-03-21
通讯作者: 林少彬.Tel:(010)50930164,E-mail:13501260565@163.com
基金资助:
环保公益专项（201309045）.

Simultaneous determination of 12 monohydroxylated polycyclic aromatic hydrocarbon metabolites in human urine by ultra-performance liquid chromatography coupled with triple quadrupole mass spectrometry

FU Hui, HU Xiaojian, CHEN Xi, LIN Shaobin

National Institute of Environmental Health, Chinese Center for Disease Control and Prevention, Beijing 100021, China

Received:2017-12-28 Online:2018-05-08 Published:2014-03-21
Supported by:
Commonweal and Environmental Protection Project of the Ministry of Environmental Protection of the People's Republic of China (No. 201309045).

摘要/Abstract

摘要：

建立了高效、准确的同时测定人体尿样中多种多环芳烃代谢物的方法。取10.0 mL尿液，酶解，经固相萃取净化浓缩、0.2 μm滤膜过滤后，以Acquity UPLC^®HSS T3（100 mm×2.1 mm，1.8 μm）为分析柱，甲醇和水为流动相，采用负离子电喷雾多反应监测模式检测样品中多环芳烃代谢物的含量。12种多环芳烃代谢物在0.04~20.0 μg/L范围内线性关系良好，相关系数>0.99，方法检出限为0.01~0.41 μg/L，平均回收率为80.0%~105%，批内RSD为1.21%~9.12%，批间RSD为4.43%~19.7%。应用该方法对淮河流域某区域的100份人体尿样进行了检测，多环芳烃代谢物的检出率为98%。该方法操作简单，灵敏度高，选择性好，可同时检测尿中12种羟基多环芳烃代谢物。

关键词: 超高效液相色谱-三重四极杆质谱, 单羟基多环芳烃代谢物, 固相萃取, 尿

Abstract:

An efficient and accurate method was developed for the simultaneous determination of 12 monohydroxylated polycyclic aromatic hydrocarbon metabolites in human urine. A 10.0-mL urine sample was hydrolyzed by enzyme and extracted using solid phase extraction. The metabolites were separated on an Acquity UPLC^®HSS T3 column (100 mm×2.1 mm, 1.8 μm) with a mobile phase comprising methanol and water. The analytes were detected by electrospray ionization (ESI)-MS/MS in negative mode and multiple reaction monitoring (MRM) mode. The linear ranges of the 12 metabolites were 0.04-20.0 μg/L with correlation coefficients above 0.99. The detection limits were in the range of 0.01-0.41 μg/L and the recoveries were 80.0%-105%. The intra-group relative standard deviations (RSDs) were less than 9.12% and the inter-group RSDs were less than 19.7%. This method was applied to the analysis of 100 urine samples and the results showed that the method was simple, sensitive and selective.

Key words: monohydroxylated metabolites of polycyclic aromatic hydrocarbons, solid phase extraction (SPE), ultra-performance liquid chromatography-triple quadrupole mass spectrometry (UPLC-MS/MS), urine

中图分类号:

O658

付慧, 胡小键, 陈曦, 林少彬. 超高效液相色谱-三重四极杆质谱法同时检测人尿中12种单羟基多环芳烃代谢物[J]. 色谱, 2018, 36(5): 487-492.

FU Hui, HU Xiaojian, CHEN Xi, LIN Shaobin. Simultaneous determination of 12 monohydroxylated polycyclic aromatic hydrocarbon metabolites in human urine by ultra-performance liquid chromatography coupled with triple quadrupole mass spectrometry[J]. Chinese Journal of Chromatography, 2018, 36(5): 487-492.

参考文献

[1] Song X J, Li H Y, Yin M M, et al. Chinese Journal of Chromatography, 2018, 36(1):51 宋晓娟, 李海燕, 尹明明, 等. 色谱, 2018, 36(1):51
[2] Wang M F, Yang L L, Hu E Y. Chinese Journal of Chromatography, 2017, 35(6):669 王美飞, 杨丽莉, 胡恩宇. 色谱, 2017, 35(6):669
[3] Feng L, Zhang S J, Zhu G H, et al. Chinese Journal of Chromatography, 2017, 35(4):466 冯利, 张胜军, 朱国华, 等. 色谱, 2017, 35(4):466
[4] Zhao B, Li Y Q, Zhang S K, et al. Chinese Journal of Chromatography, 2014, 32(9):960 赵波, 黎玉清, 张素坤, 等. 色谱, 2014, 32(9):960
[5] Li Z, Romanoff L C, Trinidad D A, et al. Anal Chem, 2006, 78(16):5744
[6] Pang Y H, Ma Y, Cui Y, et al. Journal of Environment and Health, 2012, 29(6):567 庞月红, 马芸, 崔燕, 等. 环境与健康杂志, 2012, 29(6):567
[7] Song X L, Hu X J, Lin S B. Journal of Environment and Health, 2017, 34(6):555 宋喜丽, 胡小键, 林少彬. 环境与健康杂志, 2017, 34(6):555
[8] Li Z, Romanoff L C, Trinidad D A, et al. Anal Bioanal Chem, 2014, 406(13):3119
[9] Campo L, Mercadante R, Rossella F, et al. Anal Chim Acta, 2009, 631(2):196
[10] Mattarozzi M, Musci M, Careri M, et al. J Chromatogr A, 2009, 1216(30):5634
[11] Campo L, Rossella F, Fustinoni S. J Chromatogr B, 2008, 875(2):531
[12] Romanoff L C, Li Z, Young K J, et al. J Chromatogr B, 2006, 835(1/2):47
[13] Smith C J, Huang W, Walcott C J, et al. Anal Bioanal Chem, 2002, 372(1):216
[14] Maisonnette C, Simon P, Hennion M C, et al. J Chromatogr A, 2006, 1120(1/2):185
[15] Yang H M, Wang Y S, Li J H, et al. Applied Chemical Industry, 2014, 43(5):957 杨红梅, 王永生, 黎俊宏, 等. 应用化工, 2014, 43(5):957
[16] Wang Y, Dong Y L, Fan R F, et al. Journal of Environment and Health, 2006, 23(1):76 王宇, 董玉莲, 范瑞芳, 等. 环境与健康杂志, 2006, 23(1):76
[17] You F, Zhu L, He L, et al. Chinese Journal of Analytical Chemistry, 2014, 42(12):1723 游钒, 朱岚, 何玲, 等. 分析化学, 2014, 42(12):1723
[18] Chauhan A, Bhatia T, Singh A, et al. J Chromatogr B, 2015, 985:110
[19] Ramsauer B, Sterz K, Hagedorn H W, et al. Anal Bioanal Chem, 2011, 399(2):877
[20] Chen Y S, Wang S, Yu J J, et al. Tobacco Science & Technology, 2012(4):37 陈玉松, 王昇, 余晶晶, 等. 烟草科技, 2012(4):37
[21] Fan R F, Ramage R, Wang D L, et al. Talanta, 2012, 93(11):383
[22] Zhao G, Chen Y, Wang S, et al. Talanta, 2013, 116(22):822
[23] Li X X, Cui S W, He M F, et al. Chinese Preventive Medicine, 2012, 13(4):246 李欣欣, 崔师伟, 何民富, 等. 中国预防医学杂志, 2012, 13(4):246
[24] Ding C M, Jin Y L, Lin S B. Chinese Journal of Analytical Chemistry, 2012, 40(3):397 丁昌明, 金银龙, 林少彬. 分析化学, 2012, 40(3):397
[25] Huang C F, Yan H F, Pan Z F, et al. Journal of Hygiene Research, 2010, 39(3):355 黄传峰, 闫慧芳, 潘祖飞, 等. 卫生研究, 2010, 39(3):355
[26] Zheng L, Ding C M, Hu X J, et al. Journal of Environment and Health, 2011, 28(4):348 郑磊, 丁昌明, 胡小键, 等. 环境与健康杂志, 2011, 28(4):348

超高效液相色谱-三重四极杆质谱法同时检测人尿中12种单羟基多环芳烃代谢物

Simultaneous determination of 12 monohydroxylated polycyclic aromatic hydrocarbon metabolites in human urine by ultra-performance liquid chromatography coupled with triple quadrupole mass spectrometry

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