基于微流控芯片的细胞外囊泡分离技术研究进展

doi:10.3724/SP.J.1123.2018.11045

色谱 ›› 2019, Vol. 37 ›› Issue (4): 343-347.DOI: 10.3724/SP.J.1123.2018.11045

基于微流控芯片的细胞外囊泡分离技术研究进展

廖泽荣¹, 李永瑞¹, 古乐², 雷润宏³, 苗云飞¹, 蓝鸿颖¹, 邓玉林¹, 耿利娜¹

1. 北京理工大学, 北京 100081;
2. 北京京东方传感技术有限公司, 北京 100176;
3. 北京大学第三医院肿瘤放疗科, 北京 100191

收稿日期:2018-11-30 出版日期:2019-04-08 发布日期:2015-02-14
通讯作者: 耿利娜.Tel:(010)68914607,E-mail:gln@bit.edu.cn
基金资助:
国家重大仪器专项（2012YQ04014006）.

Advances in microfluidic chip-based extracellular vesicle separation

LIAO Zerong¹, LI Yongrui¹, GU Le², LEI Runhong³, MIAO Yunfei¹, LAN Hongying¹, DENG Yulin¹, GENG Lina¹

1. Beijing Institute of Technology, Beijing 100081, China;
2. BOE Sensor Technology Group Co., Ltd, Beijing 100176, China;
3. Department of Radiation Oncology, Peking University Third Hospital, Beijing 100191, China

Received:2018-11-30 Online:2019-04-08 Published:2015-02-14
Supported by:
Specially-Funded Program on National Key Scientific Instruments and Equipment Development (No. 2012YQ04014006).

摘要/Abstract

摘要：

细胞外囊泡（extracellular vesicles，EVs）是脂质双分子层包绕形成的半球状囊泡。研究表明EVs存在重要的生物学功能，同时EVs排放的数量、种类以及内含蛋白质、脂质或RNA等构成变化与疾病密切相关。EVs的研究将有助于理解其生物学功能和作用机制，同时也有望用于疾病的诊断和治疗，因此拥有巨大的临床应用前景。从复杂的体液样品中分离捕获EVs是实现基于EVs开展医学研究以及临床诊断的前提，但是目前绝大多数的EVs分离捕获仍然是采用传统分离手段，纯度低、效率差，迫切需要高效和高选择性的EVs分离手段。先进的微流控芯片技术具有微型化、集成化和自动化的优势，利用微流控芯片的EVs分离技术研究已成热点，本文围绕相关研究的最新进展进行了综述。

关键词: 分离, 微流控芯片, 细胞外囊泡

Abstract:

Extracellular vesicles (EVs) are hemispherical vesicles that have a lipid bilayer. Studies have shown that EVs have important biological functions. The amount, types, and compositional changes of proteins, lipids and ribonucleic acids are closely related to diseases. The separation and capture of EVs from the complicated body fluid samples is a prerequisite for medical research and liquid biopsy based on EVs. However, presently the majority of EVs separation and capture still use the traditional separation methods with low purity and low efficiency. Therefore, efficient and highly selective EVs separation method is in urgent need. To meet this challenge, advanced microfluidic chip technology, which has the advantages of miniaturization, integration, and automation, can be utilized. The development of EV separation technology combined with microfluidic chips has become the focus of research. This paper summarizes the latest research progress in this area.

Key words: extracellular vesicles, microfluidic chip, separation

中图分类号:

O658

廖泽荣, 李永瑞, 古乐, 雷润宏, 苗云飞, 蓝鸿颖, 邓玉林, 耿利娜. 基于微流控芯片的细胞外囊泡分离技术研究进展[J]. 色谱, 2019, 37(4): 343-347.

LIAO Zerong, LI Yongrui, GU Le, LEI Runhong, MIAO Yunfei, LAN Hongying, DENG Yulin, GENG Lina. Advances in microfluidic chip-based extracellular vesicle separation[J]. Chinese Journal of Chromatography, 2019, 37(4): 343-347.

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基于微流控芯片的细胞外囊泡分离技术研究进展

Advances in microfluidic chip-based extracellular vesicle separation

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