人体邻苯二甲酸酯暴露的尿液生物标志物分析方法

doi:10.3724/SP.J.1123.2019.03029

摘要/Abstract

摘要： 邻苯二甲酸酯（phthalates，PAEs）是一类典型的环境内分泌干扰物。近年来，由于PAEs产量和使用量的增加，其对人体健康的危害尤其是生殖发育毒性受到了人们高度关注。由于PAEs在环境、食物（材）中广泛存在，导致人体不可避免地长期暴露于PAEs化合物，因此很有必要开展人体PAEs暴露评估。对人体尿液样品中的PAEs代谢物进行筛选和定量是评价PAEs暴露的重要手段，而建立它们准确、可靠的分析方法是重要前提。目前，邻苯二甲酸单酯和次级代谢物分别是短链和长链PAEs暴露最为常用的生物标志物。离线或在线固相萃取与高效液相色谱-串联质谱联用已成为测定PAEs代谢物的首选方法。本文主要综述了人体PAEs暴露的尿液生物标志物的分析方法，并讨论了这些方法在实际应用中的优点、局限性及挑战。

关键词: 固相萃取, 邻苯二甲酸酯暴露, 气相色谱-质谱, 生物标志物, 液相色谱-串联质谱, 综述

Abstract: Phthalates (PAEs) are a class of endocrine-disrupting chemicals. In recent years, the harmful effects of PAEs on human health, in particular their toxicity toward the reproductive system and development, have received significant attention because of their increasing production and usage. PAEs are ubiquitous in the environment and food products, resulting in unavoidable and long-term exposure in humans. Therefore, exposure and risk assessments of PAEs in humans are necessary. Screening and quantification of phthalate metabolites in urine is an important method for evaluating PAEs exposure, and establishing accurate analytical methods for this purpose has become a high priority. Phthalate monoesters and secondary metabolites are the most commonly targeted biomarkers of exposure to short-and long-chain PAEs, respectively. The combination of off-line or on-line solid phase extraction (SPE) with high performance liquid chromatography-tandem mass spectrometry has become the preferred method for the determination of phthalate metabolites. This paper reviews the analytical methods available for the detection of urinary phthalate metabolites and discusses the advantages, limitations, and challenges presented by each method in practical applications.

Key words: biomarkers, gas chromatography-mass spectrometry (GC-MS), liquid chromatography-tandem mass spectrometry (LC-MS/MS), phthalate exposure, review, solid phase extraction (SPE)

中图分类号:

O658

黄超囡, 李云, 彭俊钰, 陈吉平. 人体邻苯二甲酸酯暴露的尿液生物标志物分析方法[J]. 色谱, 2019, 37(8): 815-823.

HUANG Chaonan, LI Yun, PENG Junyu, CHEN Jiping. Analytical methods for urinary biomarkers of human exposure to phthalates[J]. Chinese Journal of Chromatography, 2019, 37(8): 815-823.

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