色谱

色谱 ›› 2019, Vol. 37 ›› Issue (8): 831-835.DOI: 10.3724/SP.J.1123.2019.04048

• 研究论文 • 上一篇    下一篇

基于MIL-100(Fe)的磁性纳米碳材料的制备及其在内源性肽富集中的应用

赵雅梦, 王敏, 张凌怡, 鲁誉浩, 张维冰   

  1. 华东理工大学化学与分子工程学院, 上海 200237
  • 收稿日期:2019-04-30 出版日期:2019-08-08 发布日期:2015-07-30
  • 通讯作者: 张维冰
  • 基金资助:
    国家自然科学基金（21475044，51472121）.

Preparation of magnetic nanoparticles based on MIL-100(Fe) and its application in the enrichment of endogenous peptides

ZHAO Yameng, WANG Min, ZHANG Lingyi, LU Yuhao, ZHANG Weibing   

  1. School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai 200237, China
  • Received:2019-04-30 Online:2019-08-08 Published:2015-07-30
  • Supported by:
    National Natural Science Foundation of China (Nos. 21475044, 51472121).

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摘要： 以Fe3O4为核，采用层层自组装的方法合成核壳型Fe3O4@MIL-100（Fe）磁性纳米材料，经高温煅烧得到具有较大孔径（17.78 nm）、高含碳量（6.79%）的磁性纳米材料Fe3O4@MC。将该材料用于多肽的富集，并通过基质辅助激光解吸电离飞行时间质谱仪（MALDI-TOF MS）进行鉴定。Fe3O4@MC材料从牛血清白蛋白（BSA）酶解液中富集得到33条肽段，且具有较高的选择性（BSA酶解液与BSA的质量比为1：400），用于人血清中内源性肽段的分离富集也取得了较好的效果。

关键词: 富集, 基质辅助激光解吸电离飞行时间质谱, 金属有机框架, 内源性肽段

Abstract: Core-shell magnetic nanoparticles, Fe3O4@MIL-100(Fe), were synthesized by layer-by-layer self-assembly. The final material, Fe3O4@MC, was obtained after high temperature calcination. Fe3O4@MC magnetic nanoparticles exhibited large pore size (17.78 nm) and high carbon content (6.79%). Thirty-three peptides of bovine serum albumin (BSA) digest were enriched by Fe3O4@MC magnetic nanomaterial. Fe3O4@MC magnetic nanomaterial also showed high selectivity for peptides when the mass ratio of BSA digest to BSA was 1:400. In addition, the nanomaterial material exhibited excellent enrichment performance for the endogenous peptides of human serum.

Key words: endogenous peptides, enrichment, matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS), metal-organic framework (MOFs)

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