色谱

色谱 ›› 2019, Vol. 37 ›› Issue (8): 904-910.DOI: 10.3724/SP.J.1123.2019.04037

• 研究论文 • 上一篇    下一篇

在线二维集束毛细管柱-高气压光电离-飞行时间质谱用于单萜类化合物的快速测量

陈璇1,2, 花磊1, 王艳3, 侯可勇1, 蒋吉春1, 谢园园1, 李海洋1   

  1. 1. 中国科学院分离分析化学重点实验室, 中国科学院大连化学物理研究所, 辽宁 大连 116023;
    2. 中国科学院大学, 北京 100049;
    3. 浙江农林大学农业与食品科学学院, 浙江 杭州 311300
  • 收稿日期:2019-04-25 出版日期:2019-08-08 发布日期:2015-07-30
  • 通讯作者: 花磊, 李海洋
  • 基金资助:
    国家重点研发计划项目（2016YFC0800902）；国家自然科学基金（21705148）.

Fast online two-dimensional analysis of monoterpenes using multi-capillary column high-pressure photoionization time-of-flight mass spectrometry

CHEN Xuan1,2, HUA Lei1, WANG Yan3, HOU Keyong1, JIANG Jichun1, XIE Yuanyuan1, LI Haiyang1   

  1. 1. Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, China;
    2. University of Chinese Academy of Sciences, Beijing 100049, China;
    3. College of Agricultural and Food Sciences, Zhejiang A & F University, Hangzhou 311300, China
  • Received:2019-04-25 Online:2019-08-08 Published:2015-07-30
  • Supported by:
    National Key Research and Development Program of China (No. 2016YFC0800902); National Natural Science Foundation of China (No. 21705148).

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摘要： 单萜类化合物是大气中生物源挥发性有机物（BVOCs）的一个主要组分，具有同分异构体种类多、寿命短、浓度低、时空变化快等特点。将集束毛细管柱（MCC）和高气压光电离-飞行时间质谱仪（HPPI-TOF MS）相结合，搭建MCC-HPPI-TOF MS联用装置，并开发一种二维GC-MS快速在线检测方法，用于单萜同分异构体的快速定性和定量分析。实验结果表明，在无任何样品预富集的前提下，该方法可在180 s内实现α-蒎烯、β-蒎烯、α-萜烯、γ-萜烯、3-蒈烯及柠檬烯等6种单萜同分异构体的在线检测，检出限（LODs）低至μg/m3量级。该方法已经用于松树和柏树枝叶释放出的单萜化合物的快速在线分析，展示了其在环境监测、过程分析等领域复杂混合物样品在线检测的能力和应用前景。

关键词: 单萜, 高气压光电离-飞行时间质谱, 集束毛细管柱, 同分异构体分析, 在线二维色谱-质谱

Abstract: One of the most abundant biological volatile organic compounds (BVOCs) in the atmosphere, monoterpene, is characterized by its short lifetime, low concentration, fast temporal and spatial variations, and wide variety of isomers. In this study, a multi-capillary column (MCC) was combined with high-pressure photoionization time-of-flight mass spectrometry (HPPI-TOF MS) and employed to develop an MCC-HPPI-TOF MS combination instrument as an online two-dimensional gas chromatography-mass spectrometry (GC-MS) method for the rapid qualitative and quantitative analysis of monoterpene isomers. As a result, six monoterpene isomers, α -pinene, β -pinene, α -terpinene, γ -terpinene, 3-carene, and limonene, were successfully isolated in 180 s with limits of detection (LODs) as low as 6 μg/m3 without sample pre-enrichment. This method was successfully applied to the rapid online analysis of monoterpenes released from the branches and leaves of Cedrus atlantica and Sabina chinensis, which shows the capability and potential application of the method for the online detection of complex sample mixtures in environmental monitoring, process analysis, and other fields.

Key words: high-pressure photoionization time-of-flight mass-spectrometry (HPPI-TOF MS), isomer analysis, monoterpene, multi-capillary column (MCC), online two-dimensional gas chromatography-mass spectrometry (GC-MS)

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