基于气相色谱-质谱的拟靶向代谢组学方法分析大米中脂肪酸

doi:10.3724/SP.J.1123.2019.05041

色谱 ›› 2020, Vol. 38 ›› Issue (2): 250-254.DOI: 10.3724/SP.J.1123.2019.05041

基于气相色谱-质谱的拟靶向代谢组学方法分析大米中脂肪酸

王希越, 明明, 连丽丽, 张浩, 娄大伟()

化学与制药工程学院, 吉林化工学院, 吉林吉林 132022

收稿日期:2019-05-26 出版日期:2020-02-08 发布日期:2020-12-10
通讯作者: 娄大伟
作者简介:娄大伟.Tel:(0432)63083163, E-mail:dwlou@hotmail.com
基金资助:
国家自然科学基金(21375046);国家自然科学基金(21605056);吉林省自然科学基金(20180101292JC)

Analysis of fatty acids in rice by pseudotargeted metabolomics method based on gas chromatography-mass spectrometry

WANG Xiyue, MING Ming, LIAN Lili, ZHANG Hao, LOU Dawei()

College of Chemistry and Pharmaceutical Engineering, Jilin Institute of Chemical Technology, Jilin 132022, China

Received:2019-05-26 Online:2020-02-08 Published:2020-12-10
Contact: LOU Dawei
Supported by:
National Natural Science Foundation of China(21375046);National Natural Science Foundation of China(21605056);Natural Science Foundation of Jilin Province(20180101292JC)

摘要/Abstract

摘要：

建立了一种基于气相色谱-质谱的拟靶向代谢组学分析方法对大米中脂肪酸进行分析，共检测到16种脂肪酸，并研究了不同大米中脂肪酸的轮廓差异。以提取到饱和脂肪酸和不饱和脂肪酸的总量为评价指标，比较了6种提取方法及4种提取溶剂对脂肪酸提取效率的影响。将该方法用于5种不同大米（稻花香、吉星、金浪子、农大、状元）中脂肪酸的分析，发现稻花香大米中脂肪酸轮廓与其他4种均有较大差异；而金浪子与农大、状元间脂肪酸差异也较大，与吉星脂肪酸轮廓较为相似。该方法简单，有较好的稳定性和准确性，可为大米品质和营养价值改善研究提供基础数据。

关键词: 气相色谱-质谱, 拟靶向代谢组学, 脂肪酸, 大米

Abstract:

A pseudotargeted metabolomics method based on gas chromatography-mass spectrometry (GC-MS) was developed for the analysis of fatty acids in rice. A total of 16 fatty acids were detected in rice. The method was used to identify the difference between fatty acid profiles in rice. The total intensity of the saturated and unsaturated fatty acids was used as the evaluation index. The effects of six extraction methods and four extraction solvents on the extraction efficiencies of the fatty acids were compared. The developed method was used to investigate the fatty acid profiles in five types of rice (Daohuaxiang, Jixing, Jinlangzi, Nongda, and Zhuangyuan). The fatty acid profile in Daohuaxiang rice was different from that in the other four species. The fatty acids in Jinlangzi and Nongda rice were markedly different, while those in Jixing and Jinlangzi rice were similar. The method is simple, stable and accurate, and it can provide basic data for the improvement of rice quality and nutritional value.

Key words: gas chromatography-mass spectrometry (GC-MS), pseudotargeted metabolomics, fatty acids, rice

王希越, 明明, 连丽丽, 张浩, 娄大伟. 基于气相色谱-质谱的拟靶向代谢组学方法分析大米中脂肪酸[J]. 色谱, 2020, 38(2): 250-254.

WANG Xiyue, MING Ming, LIAN Lili, ZHANG Hao, LOU Dawei. Analysis of fatty acids in rice by pseudotargeted metabolomics method based on gas chromatography-mass spectrometry[J]. Chinese Journal of Chromatography, 2020, 38(2): 250-254.

图/表 6

图1 不同提取方法对饱和脂肪酸和不饱和脂肪酸响应的影响(n=3)

Fig. 1 Effect of different extraction methods on the intensities of saturated fatty acids and unsaturated fatty acids (n=3) 1. derivation and extraction simultaneous by isothermal oscillation; 2. derivation and extraction simultaneous by shaker oscillation; 3. derivation and extraction simultaneous by vortex oscillation; 4. derivation following extraction by isothermal oscillation; 5. derivation following extraction by shaker oscillation; 6. derivation following extraction by vortex oscillation.

图2 不同提取溶剂对饱和脂肪酸和不饱和脂肪酸响应强度的影响(n=5)

Fig. 2 Effect of the different extraction solvents on the intensities of saturated fatty acids and unsaturated fatty acids (n=5)

图3 QC样品中16种脂肪酸的总离子流色谱图

Fig. 3 TIC chromatogram of the 16 fatty acids in the quanlity control (QC) sample

图4 C26:0、C17:1、C17:0在选择离子扫描模式和全扫描模式下的色谱图

Fig. 4 Chromatograms of C26:0, C17:1, C17:0 in SIM and full scan modes

图5 5种大米样品的主成分分析得分图

Fig. 5 Principal component analysis (PCA) score plot of the five rice samples

图6 5种大米样品中脂肪酸的热图

Fig. 6 Heat map of the fatty acids in the five rice samples

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基于气相色谱-质谱的拟靶向代谢组学方法分析大米中脂肪酸

Analysis of fatty acids in rice by pseudotargeted metabolomics method based on gas chromatography-mass spectrometry

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