色谱

色谱 ›› 2020, Vol. 38 ›› Issue (3): 278-286.DOI: 10.3724/SP.J.1123.2019.09015

• 专论与综述 • 上一篇    下一篇

N-磷酸化修饰蛋白质的富集和鉴定方法

胡晔晨1,2, 江波1, 张丽华1,*(), 张玉奎1   

  1. 1 中国科学院分离分析化学重点实验室, 中国科学院大连化学物理研究所, 辽宁 大连 116023
    2 中国科学院大学, 北京 10049
  • 收稿日期:2019-09-12 出版日期:2020-03-08 发布日期:2020-12-10
  • 通讯作者: 张丽华
  • 作者简介:张丽华, Tel:(0411)84379720, E-mail:lihuazhang@dicp.ac.cn
  • 基金资助:
    国家自然科学基金(21505133);国家自然科学基金(91753110)

Advancesin enrichment and detection methods for N-phosphorylated proteins

HU Yechen1,2, JIANG Bo1, ZHANG Lihua1,*(), ZHANG Yukui1   

  1. 1 CAS Key Laboratory of Separation Sciences for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China
    2 University of Chinese Academy of Sciences, Beijing 100049, China
  • Received:2019-09-12 Online:2020-03-08 Published:2020-12-10
  • Contact: ZHANG Lihua
  • Supported by:
    National Natural Science Foundation of China(21505133);National Natural Science Foundation of China(91753110)

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摘要：

蛋白质磷酸化修饰在细胞的信号转导、代谢、发育等生命过程中发挥着重要作用。除了研究较为透彻的发生在丝氨酸、苏氨酸和酪氨酸侧链羟基的O-磷酸化修饰之外，近年来，发生在组氨酸、精氨酸和赖氨酸侧链氨基的N-磷酸化修饰受到了越来越广泛的关注。然而，由于N-磷酸化修饰具有独特的P-N键结构，导致其化学稳定性差。尤其是在O-磷酸化肽段富集常用的酸性条件下，N-磷酸化极易丢失。因此，目前对N-磷酸化蛋白质的研究仍处于初始阶段。该文针对蛋白质N-磷酸化修饰的特点、富集和鉴定方法进行了综述，并对其发展前景进行了展望。

关键词: 蛋白质N-磷酸化修饰, 富集, 检测, 综述

Abstract:

Protein phosphorylation plays an important role in various life processes, such as signaling, metabolism, and growth. In addition to the well-known O-phosphorylation, which occurs on serine, threonine and tyrosine side chains, N-phosphorylation, occurring on histidine, arginine and lysine side chains, has attracted much attention. However, due to the chemical instability of N-phosphorylation, the studies on it are still in the nascent stage. In this review, enrichment and detection methods for protein N-phosphorylation are reviewed, and future research in this field is prospected.

Key words: protein N-phosphorylation, enrichment, detection, review