色谱

色谱 ›› 2019, Vol. 37 ›› Issue (11): 1157-1161.DOI: 10.3724/SP.J.1123.2019.04017

• 研究论文 • 上一篇    下一篇

超高效液相色谱-串联质谱法测定蛋白胨中9种B族维生素

姜莎1, 辛慧2, 莫日根2, 杜艳2, 李振莲2, 朱叶青2   

  1. 1.内蒙古自治区食品检验检测中心, 内蒙古 呼和浩特 010090;
    2.呼和浩特市食品检验所, 内蒙古 呼和浩特 010090
  • 收稿日期:2019-04-09 出版日期:2019-11-08 发布日期:2020-12-11
  • 通讯作者: 朱叶青.Tel:(0471)3457971,E-mail:hhhtzyq@sina.com.
  • 基金资助:
    科技部"食品安全关键技术研发"重点专项（2017YFC1601400）.

Determination of nine B vitamins in peptone using ultra high performance liquid chromatography-tandem mass spectrometry

JIANG Sha1, XIN Hui2, MO Rigen2, DU Yan2, LI Zhenlian2, ZHU Yeqing2   

  1. 1. Food Inspection and Testing Center of Inner Mongolia Autonomous Region, Hohhot 010090, China;
    2. Hohhot Food Inspection Institute, Hohhot 010090, China
  • Received:2019-04-09 Online:2019-11-08 Published:2020-12-11
  • Supported by:
    the Special Program for Key Technology in Food Safety of the Ministry of Science and Technology of China (No. 2017YFC1601400).

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摘要： 建立了能够同时测定蛋白胨中9种B族维生素的超高效液相色谱-串联质谱法（UPLC-MS/MS）。样品经水溶解后，滤液经Syncronis C18色谱柱分离，采用电喷雾电离源，正离子、多反应监测模式进行检测，分析时间8 min，外标法定量。结果表明，9种B族维生素在各自质量浓度范围内线性良好，相关系数（R2）均大于0.999，方法的检出限为0.09~1.67 μg/L，方法的相对标准偏差均<3%（n=6）；9种B族维生素不同添加水平下的平均回收率为80.2%~103.9%。该方法简便、准确，灵敏度高，适用于蛋白胨中B族维生素的测定。

关键词: 超高效液相色谱-串联质谱, B族维生素, 蛋白胨

Abstract: A method using ultra high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) was developed for the determination of nine B vitamins in peptone. The samples were extracted with water. The analytes were separated on a Syncronis C18 column (100 mm×2.1 mm, 1.7 μm). The nine B vitamins were detected by ESI-MS/MS under the multiple reaction monitoring (MRM) mode, and the analysis was completed in 8 min. Quantification analysis was performed by using the external standard method. The correlation coefficients (R2) of the nine B vitamins in their linear ranges were greater than 0.999. The limits of detection were 0.09-1.67 μg/L. The relative standard deviations of the method were less than 3% (n=6). The mean recoveries of the nine B vitamins were 80.2%-103.9% at different spiked levels. The method is simple, accurate and sensitive, and is suitable for the determination of the nine B vitamins in peptone.

Key words: ultra high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS), B vitamins, peptone

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