色谱 ›› 2019, Vol. 37 ›› Issue (11): 1185-1192.DOI: 10.3724/SP.J.1123.2019.04016

• 研究论文 • 上一篇    下一篇

在线富集与高效液相色谱-荧光检测法联用同时检测儿童水杯中9种双酚类物质

韩疏影, 宋易霖, 康安, 邓海山, 朱栋, 池玉梅   

  1. 南京中医药大学药学院, 江苏 南京 210023
  • 收稿日期:2019-04-10 出版日期:2019-11-08 发布日期:2020-12-11
  • 通讯作者: 池玉梅.Tel:(025)85811053,E-mail:ymchi@163.com.
  • 基金资助:
    国家自然科学基金(81303311);江苏省高校自然科学基金(17KJB360009);江苏高校优势学科建设工程资助项目(PAPD).

Determination of nine bisphenols in children's water bottle by online enrichment coupled with high performance liquid chromatography-fluorescence detection

HAN Shuying, SONG Yilin, KANG An, DENG Haishan, ZHU Dong, CHI Yumei   

  1. College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China
  • Received:2019-04-10 Online:2019-11-08 Published:2020-12-11
  • Supported by:
    National Natural Science Foundation of China (No. 81303311); Natural Science Foundation for Colleges of Jiangsu (No. 17KJB360009); Priority Academic Program Development of Jiangsu Higher Education Institutions (No. PAPD).

摘要: 基于在线富集-高效液相色谱-荧光法检测法,以C18柱分别作为富集柱和分析柱,经优化荧光波长、色谱分离和富集条件,建立了同时快速检测塑料儿童水杯浸出液中9种双酚类物质的方法。方法学考察结果显示,所建方法符合含量测定要求。9种双酚类物质在各自范围内线性关系良好,相关系数(r2)均大于0.998,检出限为0.13~66.7 ng/L。9种双酚类物质的回收率为90.7%~112.4%(RSD<11.3%,n=6)。结果显示,除双酚芴外,其他双酚类物质均有检出,浸出量随盛放时间增加而增大,高温多次浸泡后部分双酚类浸出量降低。该方法灵敏度高,操作简便,环境友好,可实现多种新型双酚类物质的同时快速检测。

关键词: 高效液相色谱-荧光检测, 在线富集, 双酚类物质, 塑料儿童水杯

Abstract: A method was developed for the rapid determination of bisphenol A (BPA) and eight structural analogs in children's plastic water bottles by online enrichment coupled with high performance liquid chromatography-fluorescence detection (HPLC-FLD). The correlation coefficients of the nine bisphenols were greater than 0.998. The limits of detection (LOQs) ranged from 0.13 ng/L to 66.7 ng/L. The recoveries ranged from 90.7% to 112.4% (RSD<11.3%, n=6). This method was applied to monitor nine bisphenols in children's water bottles. The results showed that except 4,4'-(9H-fluoren-9-ylidene)bisphenol (BPFL), all the remaining eight bisphenols were detected in different water bottles. The amounts of bisphenols leached increased with the increase of soaking time, and decreased after washing several times at 100℃. The proposed strategy is rapid, sensitive, reliable and eco-friendly, and is suitable for the simultaneous analysis of new bisphenols in water samples.

Key words: high performance liquid chromatography-fluorescence detection (HPLC-FLD), online enrichment, bisphenols, children's plastic water bottles

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