基于超高效液相色谱-高分辨质谱的多肽组学技术用于人参不同部位多肽的差异分析

doi:10.3724/SP.J.1123.2019.09006

色谱 ›› 2019, Vol. 37 ›› Issue (12): 1305-1313.DOI: 10.3724/SP.J.1123.2019.09006

基于超高效液相色谱-高分辨质谱的多肽组学技术用于人参不同部位多肽的差异分析

赵楠^1,2, 程孟春¹, 吴玉林^1,3, 刘丹¹, 张晓哲¹

1.中国科学院分离分析化学重点实验室, 中国科学院大连化学物理研究所, 辽宁大连 116023;
2.中国科学院大学, 北京 100049;
3.河南中医药大学, 河南郑州 450046

收稿日期:2019-09-06 出版日期:2019-12-08 发布日期:2020-12-11
通讯作者: 张晓哲.Tel:(0411)84379756,E-mail:zhangxz@dicp.ac.cn.
基金资助:
国家自然科学基金（81573830，81774154）.

Differential analysis of peptides in Panax ginseng C. A. Meyer root by ultra-performance liquid chromatography-high resolution mass spectrometry

ZHAO Nan^1,2, CHENG Mengchun¹, WU Yulin^1,3, LIU Dan¹, ZHANG Xiaozhe¹

1. CAS Key Laboratory of Separation Sciences for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China;
2. University of Chinese Academy of Sciences, Beijing 100049, China;
3. Henan University of Chinese Medicine, Zhengzhou 450046, China

Received:2019-09-06 Online:2019-12-08 Published:2020-12-11
Supported by:
National Natural Science Foundation of China (Nos. 81573830, 81774154).

摘要/Abstract

摘要： 采用基于超高效液相色谱-高分辨质谱的多肽组学技术对人参主根、支根、须根和芦头的多肽谱进行全面分析，旨在评价人参不同形态区域多肽表达的异同。本研究共表征62个数据库中已收录的人参多肽。结果表明，人参不同部位均富含多肽类成分。多肽组学研究发现，人参主根和支根、芦头与须根之间多肽含量具有显著差异，从鉴定到的多肽中共发现25个稳定表达的已知潜在多肽标志物。其中多肽种类及含量在主根与其他部位间差异最显著，为主根与非主根药效差异研究提供了新思路。本研究揭示了人参多肽结构多样性及人参不同部位人参多肽表达的异同，对人参化学特征评价具有重要意义，为人参质量控制和合理应用提供了化学依据。

关键词: 超高效液相色谱-高分辨质谱, 多肽, 人参, 多肽组学

Abstract: An ultra-performance liquid chromatography-high resolution mass spectrometry (UPLC-HRMS)-based peptidomics approach was developed for evaluating the similarities and differences between peptides in different morphological regions of Panax ginseng C. A. Meyer root. A total of 62 high-confidence peptides were characterized through database search, and four parts of ginseng root were all rich in peptides. The results of the differential analysis showed that the peptides were differentially expressed between the main root (MR), branch root (BR), rhizome (RH), and fibrous root (FR). Besides, a total of 25 potential peptide markers enabling the differentiation were discovered. In particular, the difference between the MR and the other parts was the most significant, and it led to a new viewpoint for the study of the difference in pharmacodynamics between the MR and the non-MR. These findings illustrate the structural diversity of ginseng peptides and reveal the similarities and differences between the peptides in ginseng root, thus providing chemical evidence for the quality control and rational application of ginseng.

Key words: ultra-performance liquid chromatography-high resolution mass spectrometry (UPLC-HRMS), peptides, Panax ginseng, peptidomics

中图分类号:

O658

赵楠, 程孟春, 吴玉林, 刘丹, 张晓哲. 基于超高效液相色谱-高分辨质谱的多肽组学技术用于人参不同部位多肽的差异分析[J]. 色谱, 2019, 37(12): 1305-1313.

ZHAO Nan, CHENG Mengchun, WU Yulin, LIU Dan, ZHANG Xiaozhe. Differential analysis of peptides in Panax ginseng C. A. Meyer root by ultra-performance liquid chromatography-high resolution mass spectrometry[J]. Chinese Journal of Chromatography, 2019, 37(12): 1305-1313.

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基于超高效液相色谱-高分辨质谱的多肽组学技术用于人参不同部位多肽的差异分析

Differential analysis of peptides in Panax ginseng C. A. Meyer root by ultra-performance liquid chromatography-high resolution mass spectrometry

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