色谱

色谱 ›› 2021, Vol. 39 ›› Issue (1): 10-14.DOI: 10.3724/SP.J.1123.2020.07042

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液相色谱梯度洗脱中的谱带压缩效应

郝卫强1,2,*(), 刘丽娟1, 沈巧银1   

  1. 1.常州工程职业技术学院, 江苏 常州 213164
    2.南京大学常州高新技术研究院, 江苏 常州 213164
  • 收稿日期:2020-08-04 出版日期:2021-01-08 发布日期:2020-12-20
  • 通讯作者: 郝卫强
  • 作者简介:

    郝卫强: 博士,研究员,1976年出生。1994年就读于中国药科大学药物分析专业,获学士和硕士学位。2001年就读于中国科学院大连化学物理研究所分析化学专业,获博士学位。2005年进入复旦大学化学系博士后流动工作站。出站后先后在南京大学常州高新技术研究院以及常州工程职业技术研究院工作。长期从事液相色谱的基础与应用研究。近年来对梯度液相色谱的分离机理展开了研究,推导得到该模式下不同色谱模型的解析解。在此基础上探讨了梯度洗脱的分离特点,并运用计算机辅助的方法对实践中梯度分析条件进行了优化。主持和参与国家、省、市项目十余项,发表论文40余篇,获中国发明专利授权4件、软件著作权5件。荣获复旦大学优秀博士后、江苏省优秀科技工作者、常州市十佳科技工作者、常州市科教城首届青年蓓蕾奖等荣誉。入选江苏省第四期、第五期“333工程”培养对象第三层次。*Tel:(0519)86339288,E-mail:hao_w_q@163.com.
  • 基金资助:
    江苏省自然科学基金(SBK2020020427);江苏省“333工程”科研项目(BRA2020310);常州工程职业技术学院校级科技创新团队建设项目

Effects of peak compression in gradient elution of liquid chromatography

HAO Weiqiang1,2,*(), LIU Lijuan1, SHEN Qiaoyin1   

  1. 1. Changzhou Vocational Institute of Engineering, Changzhou 213164, China
    2. High-Tech Research Institute of Nanjing University, Changzhou 213164, China
  • Received:2020-08-04 Online:2021-01-08 Published:2020-12-20
  • Contact: HAO Weiqiang
  • Supported by:
    Natural Science Foundation of Jiangsu Province of China(SBK2020020427);the “333 Project” Research Item of Jiangsu Province of China(BRA2020310);Sci-Tech Innovation Team Project of Changzhou Vocational Institute of Engineering.

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摘要：

谱带压缩效应是梯度洗脱区别于等度洗脱的重要特征。经典的范德姆特(van Deemter)理论塔板高度方程基于等度洗脱推导得到,因此不能对谱带压缩效应进行描述。在梯度洗脱中,保留因子(k)会随流动相组成(φ)的改变而发生变化,这就使得对梯度洗脱机理的研究要比等度洗脱复杂许多。该文对近10年来谱带压缩效应的研究进展,特别是溶剂强度模型(即描述ln k与φ关系的数学表达式)的非线性特征对谱带压缩因子(G)的影响进行了述评,指出为了更好地认识谱带压缩效应需要将这种非线性因素考虑在内。

关键词: 液相色谱, 梯度洗脱, 谱带压缩, 溶剂强度模型

Abstract:

Peak compression is a unique feature of gradient elution and is non-existent in isocratic elution. Since the classical plate height equation, which is also called as van Deemter equation, is derived by assuming isocratic elution, it cannot be used to account for the effects of peak compression. As opposed to the case of isocratic elution, the retention factor (k) varies with the mobile phase composition (φ) under gradient elution, thereby complicating mathematical analysis. Herein, the research progress on peak compression in the past decade, especially the effect of the nonlinear feature of solvent strength models (i. e., expressions for ln k vs. φ), is reviewed. A general expression for the peak compression factor (G) is introduced, for which the variation in plate height (H) with φ is ignored. Based on this equation, the classical equation for G, which was first proposed by Poppe and assumes the linear solvent strength model (LSSM) and linear gradient elution, can be derived. The effects of pre-elution of the solute in the initial mobile phase on G, which are attributed to the dwelling time of the system, are included in the Poppe equation. When the solvent strength model is nonlinear, e. g., the quadratic solvent strength model (QSSM), the analytical expressions for G can also be obtained from the general expression. Under ideal chromatographic conditions, where H=0 and the adsorption isotherm is linear, the peak compression is determined by the ratio of the retention factor of the solute in the initial mobile phase to that at the eluted mobile phase composition.

Key words: liquid chromatography (LC), gradient elution, peak compression, solvent strength model

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