基于成簇的规则间隔短回文重复序列的严重急性呼吸综合征冠状病毒2检测的最新进展

doi:10.3724/SP.J.1123.2022.08001

色谱 ›› 2022, Vol. 40 ›› Issue (9): 773-781.DOI: 10.3724/SP.J.1123.2022.08001

基于成簇的规则间隔短回文重复序列的严重急性呼吸综合征冠状病毒2检测的最新进展

周雯¹^,², 杨开广¹^,^*(), 张丽华¹^,^*(), 梁振¹, 张玉奎¹

1.中国科学院大连化学物理研究所, 中国科学院分离分析化学重点实验室, 辽宁大连 116023
2.中国科学院大学, 北京 100049

收稿日期:2022-08-01 出版日期:2022-09-08 发布日期:2022-09-26
通讯作者: *Tel:(0411)84379779,E-mail: yangkaiguang@dicp.ac.cn(杨开广);Tel:(0411)84379779,E-mail: lihuazhang@dicp.ac.cn(张丽华).
基金资助:
国家自然科学基金(21874131);国家自然科学基金(21725506);中科院“青年创新促进会”(Y2021058);中科院大连化学物理研究所创新基金(DICP I202030)

Recent advances in clustered regularly interspaced short palindromic repeats-based detection of severe acute respiratory syndrome coronavirus 2

ZHOU Wen¹^,², YANG Kaiguang¹^,^*(), ZHANG Lihua¹^,^*(), LIANG Zhen¹, ZHANG Yukui¹

1. CAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China
2. University of Chinese Academy of Science, Beijing 100049, China

Received:2022-08-01 Online:2022-09-08 Published:2022-09-26
Supported by:
National Natural Science Foundation of China(21874131);National Natural Science Foundation of China(21725506);“Youth Innovation Promotion Association” of CAS(Y2021058);Innovation Foundation of DICP, CAS(DICP I202030)

摘要/Abstract

摘要：

严重急性呼吸综合征冠状病毒2(SARS-CoV-2)导致的新冠肺炎(COVID-19)迅速蔓延全球,给全球公共卫生系统带来了挑战。由于逆转录-定量聚合酶链反应(RT-qPCR)和抗原测试的普遍适用性和灵敏度较差,并且具有不同突变的SARS-CoV-2变体持续的出现,给疫情防控带来了更大的挑战,因此,高灵敏度、无需设备并且能够区分SARS-CoV-2变体的诊断方法亟须发展。基于成簇的规则间隔短回文重复序列(CRISPR)的诊断对设备要求低,具有可编程性、灵敏性和易用性,已经发展出多种核酸检测工具用于传染病的诊断,其在临床上具有巨大的应用潜力。文章聚焦于近期发表的基于CRISPR实现SARS-CoV-2检测和变体区分的最新技术,总结其特点并对其发展进行了展望。

关键词: 严重急性呼吸综合征冠状病毒2(SARS-CoV-2), 等温核酸扩增, 成簇的规则间隔短回文重复序列(CRISPR), SARS-CoV-2变体

Abstract:

The rapid global spread of coronavirus disease 2019 (COVID-19), caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has introduced various challenges in global public health systems. The poor applicability and sensitivity of the reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and antigen-based tests, as well as the persistent emergence of SARS-CoV-2 variants with different mutations hinder satisfactory epidemic prevention and control. Therefore, there is an urgent need for diagnostic technologies capable of distinguishing SARS-CoV-2 variants with high sensitivity and low (or no) equipment dependence. Diagnosis based on clustered regularly interspaced short palindromic repeats (CRISPR) has low equipment requirements and is programmable, sensitive, and easy to use. Various nucleic acid detection tools with great clinical potential have been developed for the diagnosis of infectious diseases. Therefore, this review focuses on the reported state-of-the-art CRISPR diagnostic technologies developed for the detection and differentiation of SARS-CoV-2 variants, summarizes their characteristics and provides an outlook for their development.

Key words: severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), isothermal nucleic acid amplification, clustered regularly interspaced short palindromic repeats (CRISPR), SARS-CoV-2 variants

中图分类号:

O658

周雯, 杨开广, 张丽华, 梁振, 张玉奎. 基于成簇的规则间隔短回文重复序列的严重急性呼吸综合征冠状病毒2检测的最新进展[J]. 色谱, 2022, 40(9): 773-781.

ZHOU Wen, YANG Kaiguang, ZHANG Lihua, LIANG Zhen, ZHANG Yukui. Recent advances in clustered regularly interspaced short palindromic repeats-based detection of severe acute respiratory syndrome coronavirus 2[J]. Chinese Journal of Chromatography, 2022, 40(9): 773-781.

图/表 6

图 1 CARMEN中芯片功能详细原理图[13]

Fig. 1 Detailed schematic of loader and chip function in CARMEN[13]

图 2 CARMEN v.1和mCARMEN工作流程示意图[17]

Fig. 2 Schematic of CARMEN v.1 and mCARMEN workflows[17]

图 3 简化的mCARMEN工作流程示意图[17]

Fig. 3 Schematic of the streamlined mCARMEN workflow[17]

图 4 SHINE示意图[9]

Fig. 4 Schematic of SHINE[9]

图 5 SHINE v.2工作流程示意图[23]

Fig. 5 Schematic of the SHINE v.2 workflow[23]

图 6 ADESSO在临床样本中检测SARS-CoV-2的实验工作流程图[26]

Fig. 6 Graphic of the experimental workflow of ADESSO to detect SARS-CoV-2 in clinical samples[26]

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基于成簇的规则间隔短回文重复序列的严重急性呼吸综合征冠状病毒2检测的最新进展

Recent advances in clustered regularly interspaced short palindromic repeats-based detection of severe acute respiratory syndrome coronavirus 2

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