色谱 ›› 2024, Vol. 42 ›› Issue (11): 1059-1067.DOI: 10.3724/SP.J.1123.2024.01010

• 研究论文 • 上一篇    下一篇

气相色谱法同时测定医疗器械产品中环氧乙烷、2-氯乙醇和乙二醇残留

刘若锦, 刘宝煜, 李挥, 邵文亮, 冯毅*()   

  1. 河北省药品医疗器械检验研究院, 河北 石家庄 050227
  • 收稿日期:2024-01-12 出版日期:2024-11-08 发布日期:2024-10-22
  • 通讯作者: * Tel:(0311)69086348,E-mail:1264972031@qq.com.
  • 基金资助:
    河北省药品监督管理局科技计划项目(2022ZC1027)

Simultaneous determination of ethylene oxide, 2-chloroethanol and ethylene glycol residues in medical device products by gas chromatography

LIU Ruojin, LIU Baoyu, LI Hui, SHAO Wenliang, FENG Yi*()   

  1. Hebei Province Testing Institute for Drug and Medical Devices, Shijiazhuang 050227, China
  • Received:2024-01-12 Online:2024-11-08 Published:2024-10-22
  • Supported by:
    Science and Technology Plan Project of the Hebei Drug Administration(2022ZC1027)

摘要:

基于气相色谱法建立了同时快速测定经环氧乙烷灭菌后医疗器械产品中环氧乙烷、2-氯乙醇和乙二醇残留量的分析方法。称取2.5 g样品,加入5 mL乙醇浸提介质,在40 ℃的浸提温度下对样品中残留物质进行浸提,浸提时间为4 h。采用液体进样方式,经DB-WAX毛细管色谱柱分离,使用氢火焰离子化检测器进行测定。升温条件为40 ℃保持5 min, 40 ℃/min升温至120 ℃保持5 min, 6 ℃/min升温至200 ℃保持2 min,载气为氮气,流速为3 mL/min,采用外标法定量。环氧乙烷、2-氯乙醇和乙二醇在其相应的范围内线性关系良好,相关系数均大于0.99,检出限为0.10~0.40 μg/g,定量限为0.30~1.20 μg/g,不同加标水平下的回收率为91.08%~116.08%,相对标准偏差(n=6)为0.56%~8.45%。该分析方法灵敏、快速、准确,可用于科学有效评价环氧乙烷灭菌的一次性使用医疗器械产品中环氧乙烷相关残留物质的风险。

关键词: 医疗器械, 气相色谱法, 环氧乙烷, 2-氯乙醇, 乙二醇

Abstract:

A gas chromatography-based method was developed for the simultaneous and rapid determination of ethylene oxide (EO), 2-chloroethanol (ECH), and ethylene glycol (EG) residues in medical devices after EO sterilization. A sample weighing 2.5 g was added with 5 mL of ethanol as the extraction medium, and the residual substances in the sample were extracted at 40 ℃ for 4 h. The samples were separated on a DB-WAX capillary column (30 m×0.53 mm×1.0 μm) and determined using a hydrogen flame ionization detector. The temperature was maintained at 40 ℃ for 5 min, increased to 120 ℃ at a rate of 40 ℃/min, held for 5 min, and then increased to 200 ℃ at a rate of 6 ℃/min, held for 2 min. The flow rate of the nitrogen gas was 3 mL/min. The split ratio was 5∶1. The inlet and detector temperatures were 200 and 300 ℃, respectively. The changes in the chromatographic peak areas over time (0.5-10 h) under different temperatures (20, 30, 40, and 50 ℃) were investigated, and the optimal extraction condition was determined to be 40 ℃ for 4 h. In the experiments, quantification was performed using an external standard method. EO, ECH, and EG exhibited good peak shapes and separation effects as well as good linearity within their respective ranges. The linear correlation coefficients for EO, ECH, and EG were greater than 0.99. The limits of detection (LODs) for EO, ECH, and EG were in the range of 0.10-0.40 μg/g, and the limits of quantification (LOQs) were in the range of 0.30-1.20 μg/g. The average recoveries under different spiked levels were in the range of 91.08%-116.08%, and the relative standard deviations (n=6) were in the range of 0.56%-8.45%. EO, ECH, and EG residues were found to exist at different levels in the medical devices tested. In particular, disposable infusion sets must be paid careful attention. ECH and EG were not detected in disposable sterile medical devices made of non-polyvinyl chloride materials, which may be due to the fact that the products themselves did not contain chloride ions, they were not exposed to chlorine-containing substances during their production, sterilization, storage, transportation, use, etc. This study established a method to detect EO residues in disposable medical devices, and has the advantages of simple operation, excellent specificity, accurate quantification, and good reproducibility. It can simultaneously detect three residual substances in medical devices while meeting the actual detection requirements for EO, ECH, and EG residues. The method can be used to scientifically and effectively evaluate the risk of EO residues in single-use medical devices sterilized with EO, and will be helpful for improving the quality of medical devices, ensuring the safety of device use, and providing a reference for regulatory supervision and testing.

Key words: medical devices, gas chromatography (GC), ethylene oxide (EO), 2-chloroethanol (ECH), ethylene glycol (EG)

中图分类号: