色谱

色谱

• 研究论文 • 上一篇    下一篇

高效阴离子交换色谱-脉冲安培检测法测定烤烟中的水溶性葡萄糖、果糖和蔗糖

王荔1,陈巧珍1,宋国新1,沈轶2,刘百战2

  

  1. 1.Center of Analysis & Measurement, Fudan University, Shanghai 200433, China;2.Shanghai Tobacco (Group) Corporation, Shanghai 200082, China
  • 收稿日期:2005-05-23 修回日期:1900-01-01 出版日期:2006-03-30 发布日期:1987-06-25
  • 通讯作者: 王荔

Determination of Water-Soluble Glucose, Fructose and Sucrose in Flue-Cured Tobacco by High Performance Anion Exchange
Chromatography Coupled with Pulsed Amperometric Detection

WANG Li1, CHEN Qiaozhen1, SONG Guoxin1, SHEN Yi2, LIU Baizhan2

  

  1. 1.Center of Analysis & Measurement, Fudan University, Shanghai 200433, China;2.Shanghai Tobacco (Group) Corporation, Shanghai 200082, China
  • Received:2005-05-23 Revised:1900-01-01 Online:2006-03-30 Published:1987-06-25
  • Contact: WANG Li

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摘要：

用高效阴离子交换色谱-脉冲安培检测法（HPAEC-PAD）测定了烤烟中的水溶性葡萄糖、果糖和蔗糖。采用水浸取及膜过滤法处理烤烟样品,以Dionex CarboPac PA-1阴离子交换柱为色谱柱，0.2 mol/L NaOH水溶液为淋洗液进行分离测定。葡萄糖、果糖和蔗糖的含量与其峰面积的线性关系良好,回收率均在97%以上。方法简便易行,灵敏度高,重现性良好，可以实现对烟草中单糖的快速分离和测定。

关键词: 高效阴离子交换色谱, 果糖, 烤烟 , 脉冲安培检测法, 葡萄糖, 蔗糖

Abstract:

The quantitative determination of water-soluble sugars such as glucose, fructose and sucrose in flue-cured tobacco by high performance anion exchange chromatography coupled with pulsed amperometric detection （HPAEC-PAD） was developed. The method was used to determine flue-cured tobacco samples containing large amounts of carbohydrates after pretreatment by water-leaching and membrane filtration. The separation was performed on a Dionex CarboPac PA-1 anion exchange column with 0.2 mol/L NaOH solution as the eluent at a flow rate of 1.0 mL/min. The injection volume was 20 μL. Good linearities were obtained for glucose, fructose and sucrose within the range of 0.5-100 mg/L (r2＞0.997). The detection limits (S/N= 3) were 0.1 mg/L, 0.1 mg/L and 0.2 mg/L, and the average recoveries were 97.3%, 101.4% and 98.9% with good reproducibility for glucose, fructose and sucrose, respectively.

Key words: flue-cured tobacco , fructose, glucose, pulsed amperometric detection (PAD), sucrose, high performance anion exchange chromatography (HPAEC)