Abstract: A reversed-phase high performance liquid chromatographic (RP-HPLC) method with dual wavelength detection was developed for the determination of vitamin C, paracetamol, chlorphenamine maleate and chlorogenic acid in Vitamin C Yinqiao Tablets. The separation was performed on a Sinochrom ODS-BP column with the mobile phase consisting of 0.05 mol/L KH2PO4 (pH 3.0, containing 1% triethylamine and acetonitrile (75:25, v/v). The detection wavelength was set at 260 nm (λ1) and 326 nm (λ2). The method linearities were wide and the average recoveries were more than 99.4%. The relative standard deviations were less than 1.8% (n=5). This method is convenient, rapid and accurate. It is readily applied to the quality controls in the production process of Vitamin C Yinqiao Tablets.

Key words: chlorogenic acid, chlorphenamine maleate, dual detection wavelengths, paracetamol, vitamin C, Vitamin C Yinqiao Tablets , high performance liquid chromatography (HPLC)