Abstract: A high performance liquid chromatographic (HPLC) method was established for the enantiomeric separation of azelnidipine. Baseline chiral separation of azelnidipine was achieved under normal-phase chromatographic mode using the Chiralpak AD-H chiral column (250 mm×4.6 mm, 5.0 μm, Daicel). The influences of the nature of chiral stationary phase, form of the mobile phase, and column temperature on the enantiomeric separation were studied. The optimized chromatographic conditions were hexane-isopropyl alcohol (90:10, v/v) as the mobile phase with a flow rate of 0.8 mL/min and detection at the wavelength of 254 nm. The column temperature was set at 20 ℃. The resolution of 3.3 for azelnidipine was achieved under the above chromatographic conditions. The method is simple, rapid and with good reproducibility.

Key words: azelnidipine , chiral split, chiral stationary phase, high performance liquid chromatography (HPLC)