Abstract: Based on the high activity of epoxy group of glycidyl methacrylate monolith, eremomycin-bonded chiral stationary phase (CSP) which has 22 different types of chiral centers was prepared by a one-step derivatization process. The preparation conditions were optimized and the preparation can be carried out in a wide pH range (6.0~9.0). The optimized concentration of eremomycin reactant solution was 42 g/L. The optimized method was simple and mild. Prepared CSP was evaluated by separating racemic mixtures of five amino acids and a chiral drug (rosiglitazone) in capillary electrochromatography mode and all of enantiomers were baseline separated. Analysis time of each pair of enantiomers was less than 4 min, so that rapid analysis can be achieved. The effects of organic modifier, buffer pH, and buffer concentration on the separation were investigated. It shows that eremomycin-bonded CSP has good chiral recognition ability. Chiral recognition mechanisms of different solutes on eremomycin-bonded CSP are discussed.

Key words: capillary electrochromatography (CEC), enantiomers, macrocyclic antibiotics, monolithic chiral stationary phase, eremomycin