Chinese Journal of Chromatography

Chinese Journal of Chromatography ›› 2010, Vol. 28 ›› Issue (11): 1020-1025.DOI: 10.3724/SP.J.1123.2010.01020

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Simultaneous determination of six synthetic sweeteners in food by high performance liquid chromatography-tandem mass spectrometry

LIU Xiaoxia1,2, DING Li1, LIU Jinxia2, ZHANG Ying1*, HUANG Zhiqiang1, WANG Libing1, CHEN Bo2   

  1. 1. Hunan Academy of Inspection and Quarantine, Technical Center of Hunan Entry-Exit Inspection and Quarantine Bureau, Hunan Key Laboratory of Food Safety Science & Technology, Changsha 410004, China; 2. Key Laboratory of Chemical Biology & Traditional Chinese Medicine Research, Ministry of Education, Hunan Normal University, Changsha 410081, China
  • Received:2010-07-21 Revised:2010-09-28 Online:2010-11-28 Published:2010-11-28

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Abstract: A simple and sensitive method for the determination of six synthetic sweeteners (sodium cyclamate, saccharin sodium, acesulfame-K, aspartame, alitame and neotame) in food was developed. The synthetic sweeteners were extracted by methanol-water (1:1, v/v). The extract was separated on a C18 column using 0.1% (v/v) formic acid-5 mmol/L ammonium formate/acetonitrile as mobile phase, and then detected by high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) using multiple reaction monitoring (MRM) mode. The good linearities (r＞0.998) were achieved for all the analytes over the range of 20~500 μg/L. The recoveries obtained ranged from 81.3% to 106.0% at three spiked concentrations, with the relative standard deviations lower than 11%. The established method has been successfully applied to the determination of synthetic sweeteners in food.

Key words: acesulfame-K, alitame, aspartame, food, neotame, saccharin sodium, sodium cyclamate, high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS)