Abstract: In order to obtain quality control samples for the analysis of flumequine residue in eel which are consistent with real detection samples, minced eel was prepared as natural matrix standard samples containing flumequine. The results showed that through the administration of drug application and appropriate time of sample acquisition, the natural positive matrix containing the expected levels (5~10 μg/kg) of flumequine and the representative of actual samples at the original state could be obtained. The uniformity of the material was ensured by homogenizing and paddle type blending. The irradiation of Co60-γ rays at 12 kGy and the packaging material could prevent the degradation of the minced samples at ambient temperature and facilitate the inter-laboratory transfer in any season.

Key words: minced eel, natural matrix standard, preparation, flumequine residue