Chinese Journal of Chromatography

Chinese Journal of Chromatography ›› 2012, Vol. 30 ›› Issue (04): 419-422.

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Determination of glyphosate in heart blood of corpse by ion chromatography

WANG Yong1, WU Bo1, LIAN Houbin2, SHI Chaoou3*   

  1. 1. Institute of Forensic Science and Technology of Nanjing Public Security Bureau, Nanjing 210001, China; 2. Gulou Substation of Nanjing Public Security Bureau, Nanjing 210036, China; 3. Analysis and Research Center, East China University of Science and Technology, Shanghai 200237, China
  • Received:2011-12-19 Revised:2012-02-26 Online:2012-04-28 Published:2012-04-28

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Abstract: A method for the determination of glyphosate in human blood by ion chromatography was established. The protein in heart blood from a corpse was precipitated with acetonitrile. The large molecules and Cl~in the supernatant were removed by a Dionex OnGuard II RP column and a Dionex OnGuard II Ag column, respectively. The filtrate was separated on an IonPac AS-19 column with KOH solution as eluent produced online by an eluent generator (EG). A suppressor with external water mode and a conductivity detector for the detection were used. The linear range of this method was 10~100 mg/L with a correlation coefficient (r2) of 0.999 9. The limits of detection (LOD, S/N=3) and quantification (LOQ, S/N=10) of glyphosate in blood were 0.12 mg/L and 0.39 mg/L, respectively. The recoveries ranged between 95.2%~109.1% with the relative standard deviations (RSDs, n=5) of 1.2%~3.7%. The glyphosate content in a heart blood sample from a corpse in an actual case was 508 mg/L detected by this method. This method is simple, sensitive, accurate, and can rapidly provided reliable clues and evidences for glyphosate poisoning cases. This method can meet the needs of public security work.

Key words: conductivity detection, corpse, glyphosate, heart blood, ion chromatography (IC)