Simultaneous determination of ephedrine and N-methylephedrine in urine by solid phase extraction-ultra performance liquid chromatography-electrospray ionization tandem mass spectrometry

doi:10.3724/SP.J.1123.2013.02028

Abstract

Abstract:

A rapid and sensitive method has been developed for the simultaneous determination of ephedrine and N-methylephedrine in urine samples by solid phase extraction-ultra performance liquid chromatography coupled with electrospray ionization tandem mass spectrometry (SPE-UPLC-ESI MS/MS). The samples were extracted with Oasis MCX solid phase extraction cartridges and measured in the modes of electrospray positive ionization (ESI+) and multiple reaction monitoring (MRM). Good linearities were observed in the range of 0.0250-2.50 μg/L with correlation coefficient over 0.9990 for both analytes. The recoveries were above 80% with RSDs less than 5.0%. The limits of detection were 0.01 μg/L. The method proves to be rapid and sensitive for the trace determination of ephedrine and N-methylephedrine in urine samples.

Key words: N-methylephedrine, ephedrine, solid phase extraction (SPE), ultra performance liquid chromatography-electrospray ionization tandem mass spectrometry (UPLC-ESI MS/MS), urine

CLC Number:

O658

ZHANG Lin, ZHANG Fucheng, WANG Zhaohong, JIANG Ye, XU Meng, LI Hong. Simultaneous determination of ephedrine and N-methylephedrine in urine by solid phase extraction-ultra performance liquid chromatography-electrospray ionization tandem mass spectrometry[J]. Chinese Journal of Chromatography, 2013, 31(9): 898-902.

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