Systematic evaluation of retention behavior of carbohydrates in hydrophilic interaction liquid chromatography

doi:10.3724/SP.J.1123.2013.06032

Abstract

Abstract:

A systematic evaluation of retention behavior of carbohydrates in hydrophilic interaction liquid chromatography (HILIC) was performed. The influences of mobile phase, stationary phase and buffer salt on the retention of carbohydrates were investigated. According to the results, the retention time of carbohydrates decreased as the proportion of acetonitrile in mobile phase decreased. Increased time of carbohydrates was observed as the concentration of buffer salt in mobile phase increased. The retention behavior of carbohydrates was also affected by organic solvent and HILIC stationary phase. Furthermore, an appropriate retention equation was used in HILIC mode. The retention equation lnk=a+blnC_B+cC_B could quantitatively describe the retention factors of carbohydrates of plant origin with good accuracy: the relative error of the predicted time to actual time was less than 0.3%. The evaluation results could provide guidance for carbohydrates to optimize the experimental conditions in HILIC method development especially for carbohydrate separation.

Key words: carbohydrate, hydrophilic interaction liquid chromatography (HILIC), retention behavior, retention equation

CLC Number:

O658

FU Qing, WANG Jun, LIANG Tu, XU Xiaoyong, JIN Yu. Systematic evaluation of retention behavior of carbohydrates in hydrophilic interaction liquid chromatography[J]. Chinese Journal of Chromatography, 2013, 31(11): 1051-1056.

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