Determination of 21 phenolic compounds in soil by ultrasonic extraction-gas chromatography

doi:10.3724/SP.J.1123.2013.05005

Abstract

Abstract:

An effective method was developed to determine phenolic compounds in soil by ultrasonic extraction-gas chromatography (GC). The soil sample was extracted with methylene dichloride-hexane (2:1, v/v) for 3 min with the extraction temperature lower than 50 ℃ for 3 times. The extract was purified by alkaline aqueous solution (pH >12), and the phenolic compounds were dissolved in aqueous solution. After the organic solution was discarded, the aqueous solution was adjusted to pH<3 and subsequently extracted with methylene dichloride-ethyl acetate (4:1, v/v) twice. The analytes were determined by GC-FID and quantified by external standard method. The limits of quantification for all tested phenolic compounds were 0.01-0.06 mg/kg in about 10 g soil samples. The recoveries were between 62.9% and 111.4% with the relative standard deviations (RSDs) in the range of 4.3%-24.0% (n=6). The results showed that this method is sensitive, accurate, and suitable for the determination of the 21 phenolic compounds in soil.

Key words: gas chromatography (GC), phenolic compounds, soil, ultrasonic extraction

CLC Number:

O658

YANG Lili, WANG Meifei, HU Enyu, LIU Jing, WU Lijuan. Determination of 21 phenolic compounds in soil by ultrasonic extraction-gas chromatography[J]. Chinese Journal of Chromatography, 2013, 31(11): 1081-1086.

References

[1] HJ 503-2009
[2] Chen H, Zhang H Y, Gai L J. Guangdong Chemical Industry (陈辉, 张红医, 盖丽娟. 广东化工), 2010, 37 (6): 102
[3] EPA Method 8540
[4] Fiehn O, Jekel M. J Chromatogr A, 1997, 769(2): 189
[5] Zhang X J, Wu R A. Chinese Journal of Chromatography (张学俊, 吴仁安. 色谱), 1998, 16(6): 530
[6] Wu X L, Zhu A L. Chinese Journal of Chromatography (吴宪龙, 朱爱丽. 色谱), 1998, 16(6): 536
[7] Sun A L, Li J, Liu R M. J Sep Sci, 2006, 29(7): 995
[8] Zhang H H, Shi R, Zhao R, et al. World Pesticides (张辉辉, 石锐, 赵蕤, 等. 世界农药), 2011, 33(3): 50
[9] Liang Y, Wang B Y. Journal of Anhui Agricultural Sciences (梁焱, 王碧云. 安徽农业科学), 2011, 39(26): 16076
[10] Zhang S Q, He G, Zhang L, et al. Journal of Southwest China Normal University: Natural Science Edition (张淑琼, 何刚, 张丽, 等. 西南师范大学学报: 自然科学版), 2011, 36(4): 50
[11] Xie X, Li Y, Chen J P. Chinese Journal of Chromatography (谢昕, 李云, 陈吉平. 色谱), 2011, 29(1): 49
[12] Wei C, Lu H J, Chen M L, et al. Chinese Journal of Chromatography (魏超, 卢珩俊, 陈梅兰, 等. 色谱), 2011, 29(1): 54
[13] Zhou W Q, Shan J, Guo H Y, et al. Journal of Nanjing University: Natural Sciences (周文强, 单军, 郭红岩, 等. 南京大学学报: 自然科学), 2012, 48(6): 768
[14] Zhu F, Tu M Z, Wang L L, et al. Journal of Instrumental Analysis (朱峰, 涂貌贞, 王丽莉, 等. 分析测试学报), 2013, 32(3): 336
[15] Zhai C H. Environmental Chemistry (翟晨淏. 环境化学), 2013, 32(1): 170
[16] EPA Method 8041A
[17] Wang J P. Chinese Journal of Chromatography (王京平. 色谱), 2004, 22(5): 562
[18] Zhou F R, Li X J, Zeng Z R. Anal Chim Acta, 2005, 538 (1/2): 63
[19] Wang H Y, Wang A Q, Zhou M. Environmental Protection Science (王红云, 王安群, 周敏. 环境保护科学), 2008, 34(4): 58
[20] Li J, Wang H. The Administration and Technique of Environmental Monitoring (李娟, 王荟. 环境监测管理与技术), 2012, 24(6): 46
[21] Zhu M H. Chinese Journal of Health Laboratory Technology (朱铭洪. 中国卫生检验杂志), 2013, 23(5): 1129
[22] Gui J Y, Zhang L, Chen Z Y, et al. Physical Testing and Chemical Analysis Part B: Chemical Analysis (桂建业, 张莉, 陈宗宇, 等. 理化检验:化学分册), 2012, 48(4): 423
[23] Faraji H. J Chromatogr A, 2005, 1087(1/2): 283
[24] Yang L L, Hu E Y, Mu Y F, et al. Environmental Monitoring in China (杨丽莉, 胡恩宇, 母应锋, 等. 中国环境监测), 2007, 23(4): 40
[25] Xiao Y F, Liu G M, Dong Y L, et al. Rock and Mineral Analysis (肖宇芳, 刘广民, 董永亮, 等. 岩矿测试), 2008, 27(2): 117
[26] Xiao Z L, Cong Q, Qu J, et al. Physical Testing and Chemical Analysis Part B: Chemical Analysis (肖振林, 丛俏, 曲蛟, 等. 理化检验:化学分册), 2010, 46(6): 660
[27] Zhou Y S, Wang X, Wang X M. The Administration and Technique of Environmental Monitoring (周延生, 王晓, 王兴民. 环境监测管理与技术), 2009, 21(3): 45
[28] Wei M C, Jen J F. J Chromatogr A, 2003, 1012: 111
[29] Mahugo Santana C, Sosa Ferrera Z, Santana Rodriguez J J. Anal Bioanal Chem, 2005, 382: 125
[30] Crespín M A, Gallego M, Valcárcel M. J Chromatogr A, 2000, 897: 279
[31] Cristina M S, Zoraida S F. Molecules, 2009, 14: 298
[32] HJ/T 166-2004
[33] EPA Method 3650B