Simultaneous determination of 25 illegally added drugs in diet health foods by ultra-high performance liquid chromatography

doi:10.3724/SP.J.1123.2013.10015

Abstract

Abstract:

An analytical method using ultra-high performance liquid chromatography (UPLC) was developed for qualitative and quantitative analysis of 25 illegally added drugs in diet health foods. The diet food samples were extracted using 40 mL methanol by sonication. After centrifugation, the supernatants were separated on a Waters HSS T3 column with gradient elution at a flow rate of 0.3 mL/min, coupling with diode array detection (DAD) in wavelength range from 200 nm to 400 nm. The binary mobile phase was acetonitrile and 10 mmol/L ammonium acetate solution (containing 0.1% formic acid). The correlation coefficient of standard curve for each drug in linearity range was not less than 0.997, as well as the recoveries of all the drugs in diet health foods were 70.7%-104% with the relative standard deviations (RSDs) of 0.132%-5.03% at three spiked levels. Seventeen diet food samples were tested, in which phenolphthalein was found in three samples and emodin was found in one sample. The method is specific, easy, quick, and suitable for confirmation of the 25 illegally added drugs in diet health foods.

Key words: diet health foods, illegally added drugs, ultra-high performance liquid chromatography (UPLC)

CLC Number:

O658

WANG Jingwen, HUANG Xianglu, CAO Jin, WANG Gangli, ZHANG Qingsheng, DING Lixia. Simultaneous determination of 25 illegally added drugs in diet health foods by ultra-high performance liquid chromatography[J]. Chinese Journal of Chromatography, 2014, 32(2): 151-156.

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