Simultaneous determination of 16 organic acids in feed additives by on-line enrichment and ion chromatography-mass spectrometry

doi:10.3724/SP.J.1123.2013.10003

Abstract

Abstract:

A novel analytical method for simultaneous determination of sixteen organic acids by on-line enrichment and ion chromatography-mass spectrometry (IC-MS) was developed. On-line enrichment and separation of the organic acids were performed by ion chromatography on a homemade enrichment column and a homemade separation column. The qualitative and quantitative analyses of the organic acids were performed by mass spectrometry in selected ion monitoring (SIM) mode on the basis of atmospheric pressure chemical ionization (APCI) source in negative mode. The sample of 200 μL was injected for the analysis, and the on-line enrichment time was 3 min. The sodium hydroxide solution was used as a gradient elution system. The two columns made it possible to have a low limit of detection due to the good enrichment and separation capability. The sixteen organic acids were separated completely within 30 min. All curves showed good linearity within the test concentration ranges. The limits of detection (LODs) were between 0.01 and 0.22 mg/L, and the average recoveries were between 70.6% and 110.8%. The relative standard deviations (RSDs) were less than 6.3%. The results indicate that this method is simple, rapid, sensitive and accurate for the determination of the organic acids in feed additives.

Key words: feed additives, ion chromatography-mass spectrometry (IC-MS), on-line enrichment, organic acids

CLC Number:

O658

XIONG Zhiyu, DONG Ying, ZHOU Hongbin, YU Yang, LI Jing, SUN Li. Simultaneous determination of 16 organic acids in feed additives by on-line enrichment and ion chromatography-mass spectrometry[J]. Chinese Journal of Chromatography, 2014, 32(2): 145-150.

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