Determination of 12 bisphenol substances in functional foods by QuEChERS and high performance liquid chromatography-tandem mass spectrometry

doi:10.3724/SP.J.1123.2014.06037

Abstract

Abstract:

A method based on high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) for the simultaneous determination of 12 bisphenol substances in functional foods (powder, tablet, capsule) was presented. The samples were extracted by acetonitrile containing 1% (v/v) acetic acid followed by further cleaned up using matrix solid-phase dispersion to remove matrix interferences. The separation of the 12 bisphenol substances was performed on a Thermo Aquasil C₁₈ column (150 mm×4.6 mm, 3.0 μm), and determined in the positive and negative MRM modes by MS/MS using matrix-matched external standard method. The results demonstrated that the calibration curves were of good linearity with the correlation coefficients greater than 0.99. The limits of detection (LODs, S/N>3) were in the range of 0.1-0.5 μg/kg and the limits of quantitation (LOQs, S/N>10) were 0.4-1.7 μg/kg. The recoveries of the 12 bisphenol substances spiked at three levels (2.0, 5.0 and 10.0 μg/kg) in matrix ranged from 60.5% to 116.3% with the relative standard deviations (RSDs) of 6.8% to 11.2%. The established method is simple, time-saving and sensitive. It can meet the requirements for current regulations while achieving qualitative and quantitative determination of the 12 bisphenol substances in functional foods.

Key words: bisphenol substances, functional food, high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS), QuEChERS

CLC Number:

O658

GAO Mengjie, ZHOU Yao, SHENG Yonggang, ZHAO Shanzhen, DENG Xiaojun, GUO Dehua, DING Zhuoping, WANG Guomin, PENG Tao. Determination of 12 bisphenol substances in functional foods by QuEChERS and high performance liquid chromatography-tandem mass spectrometry[J]. Chinese Journal of Chromatography, 2014, 32(11): 1201-1208.

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