An automatic and sensitive method for the determination of endogenous brassinosteroids in plant tissues by an online trapping-in situ derivatization-ultra performance liquid chromatography-tandem mass spectrometry system

doi:10.3724/SP.J.1123.2014.08010

Abstract

Abstract:

Brassinosteroids (BRs) are a class of naturally occurring phytohormones with polyhydroxy steroid structure, which regulate general plant growth and many physiological processes. The reported methods for BR analysis were complicated, and the detection sensitivity was relatively low. To realize the automatic analysis of trace BRs in limited plant tissues, an in-tube solid phase microextraction-ultra performance liquid chromatography-tandem mass spectrometry (SPME-UPLC-MS/MS) system was constructed based on two valves-two pumps. Using C18 PEEK column as the trapping column and 4-(dimethylamino)phenylboronic acid (4-DMAPBA) as the derivatization reagent, an on line trapping and in situ derivatization assay method of BRs was developed. BRs could be programmed to fulfill the procedures of injection, extraction, derivatization, LC separation and MS detection in the system. The detection limits of BRs were improved more than one order of magnitude by the online trapping and in situ derivatization techniques, thus endogenous BRs could be quantified in only 300 mg plant tissues.

Key words: brassinosteroid, in situ derivatization, online trapping, plant tissues, ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS)

CLC Number:

O658

DING Jun, JIANG Li, FENG Yuqi. An automatic and sensitive method for the determination of endogenous brassinosteroids in plant tissues by an online trapping-in situ derivatization-ultra performance liquid chromatography-tandem mass spectrometry system[J]. Chinese Journal of Chromatography, 2014, 32(10): 1094-1103.

References

[1] Bajguz A, Tretyn A. Phytochem, 2003, 62(7): 1027
[2] Mitchell J W, Mandava N, Worley J F, et al. Nature, 1970, 225(5237): 1065
[3] Grove M D, Spencer G F, Rohwedder W K, et al. Nature, 1979, 281(5728): 216
[4] Ashraf M, Akram N A, Arteca R N, et al. Crit Rev Plant Sci, 2010, 29(3): 162
[5] Clouse S D, Sasse J M. Annu Rev Plant Physiol Plant Mol Biol, 1998, 49: 427
[6] Mertens R, Deus-Neumann B, Weiler E W. FEBS Lett, 1983, 160(1/2): 269
[7] Yokota T, Watanabe S, Ogino Y, et al. J Plant Growth Regul, 1990, 9(1/2/3/4): 151
[8] Swaczynová J, Novák O, Hauserová E, et al. J Plant Growth Regul, 2007, 26(1): 1
[9] Bajguz A. J Plant Physiol, 2009, 166(17): 1946
[10] Schrick K, Mayer U, Martin G, et al. Plant J, 2002, 31(1): 61
[11] Khripach V A, Zhabinskii V N, Litvinovskaya R P, et al. Immunoassays of Brassinosteroids//Hayat S, Ahamd A. Brassinosteroids: A Class of Plant Hormone. Netherlands: Springer, 2011: 375.[2014-08-13]. http://link.springer.com/chapter/10.1007/978-94-007-0189-2_14
[12] Yokota T, Watanabe S, Ogino Y, et al. J Plant Growth Regul, 1990, 9(1): 151
[13] Horgen P A, Nakagawa C H, Irvin R T. Can J Biochem Cell Biol, 1984, 62(8): 715
[14] Gupta D, Bhardwaj R, Nagar P K, et al. Plant Growth Regul, 2004, 43(2): 97
[15] Takatsuto S, Ying B, Morisaki M, et al. J Chromatogr A, 1982, 239: 233
[16] Sasse J M. Physiol Plant, 1997, 100(3): 696
[17] Winter J, Schneider B, Meyenburg S, et al. Phytochem, 1999, 51(2): 237
[18] Gamoh K, Takatsuto S. Anal Chim Acta, 1989, 222(1): 201
[19] Gamoh K, Sawamoto H, Kakatsuto S, et al. J Chromatogr A, 1990, 515: 227
[20] Svatos A, Antonchick A, Schneider B. Rapid Commun Mass Spectrom, 2004, 18(7): 816
[21] Huo F, Wang X, Han Y, et al. Talanta, 2012, 99: 420
[22] Wu Q, Wu D, Shen Z, et al. J Chromatogr A, 2013, 1297: 56
[23] Xin P, Yan J, Fan J, et al. Plant Physiol, 2013, 162(4): 2056
[24] Zhang H J, Wu J H, Feng Y Q. Journal of Analytical Science (张慧娟, 吴剑虹, 冯钰锜. 分析科学学报), 2008, 24(5): 589
[25] Atapattu S N, Rosenfeld J M. J Chromatogr A, 2013, 1296: 204
[26] Awan M A, Fleet I, Thomas C L P. Food Chem, 2008, 111(2): 462
[27] Ito R, Kawaguchi M, Honda H, et al. J Chromatogr B, 2008, 872(1/2): 63
[28] Magi E, Di Carro M, Liscio C. Anal Bioanal Chem, 397(3): 1335
[29] Zhang H J, Huang J F, Lin B, et al. J Chromatogr A, 2007, 1160(1/2): 114
[30] Park Y K, Choi K, Ahmed A Y B H, et al. J Chromatogr A, 1217(20): 3357
[31] Salvador A, Moretton C, Piram A, et al. J Chromatogr A, 2007, 1145(1/2): 102
[32] Risticevic S, Niri V, Vuckovic D, et al. Anal Bioanal Chem, 2009, 393(3): 781
[33] Fan Y, Feng Y Q, Da S L, et al. Anal Chim Acta, 2004, 523(2): 251
[34] Nie J, Zhang M, Fan Y, et al. J Chromatogr B, 2005, 828(1/2): 62
[35] Liang B H, Wen Y, Lü Y N, et al. Journal of Instrumental Analysis (梁炳焕, 文毅, 吕永宁, 等. 分析测试学报), 2008, 27(1): 18
[36] Huang J F, Lin X H. Chinese Pharmaceutical Journal (黄京芳, 林幸华. 中国药学杂志), 2009, 44(12): 941
[37] Fan Y, Feng Y Q, Zhang J T, et al. J Chromatogr A, 2005, 1074(1/2): 9
[38] Wei F, Fan Y, Zhang M, et al. Electrophoresis, 2005, 26(16): 3141
[39] Lin B, Zheng M M, Ng S C, et al. Electrophoresis, 2007, 28(15): 2771
[40] Ding J, Mao L J, Wang S T, et al. Phytochem Anal, 2013, 24(4): 386
[41] Springsteen G, Wang B H. Tetrahedron, 2002, 58(26): 5291