Progress in quantitative methods based on liquid chromatography-mass spectrometry for drug metabolizing enzymes in human liver microsomes

doi:10.3724/SP.J.1123.2015.03023

Abstract

Abstract:

Cytochrome P450 (CYP) enzymes and uridine 5-diphospho-glucuronosyltransferase (UGT) enzymes are critical enzymes for drug metabolism. Both chemical drugs and traditional Chinese medicines are converted to more readily excreted compounds by drug metabolizing enzymes in human livers. Because of the disparate expression of CYP and UGT enzymes among different individuals, accurate quantification of these enzymes is essential for drug pharmacology, drug-drug interactions and drug clinical applications. The research progress in quantitative methods based on liquid chromatography-mass spectrometry for drug metabolizing enzymes in human liver microsomes in the recent decade is reviewed.

Key words: drug metabolizing enzymes, liquid chromatography-mass spectrometry (LC-MS), quantitative methods, review

CLC Number:

O658

WANG Huanhuan, LÜ Yayao, PENG Bo, QIAN Xiaohong, ZHANG Yangjun. Progress in quantitative methods based on liquid chromatography-mass spectrometry for drug metabolizing enzymes in human liver microsomes[J]. Chinese Journal of Chromatography, 2015, 33(6): 553-557.

References

[1] Evans W E, Relling M V. Science, 1999, 286: 487

[2] Zhou S F, Zhou Z W, Huang M. Toxicology, 2010, 278(2): 165

[3] Bu M H, Zheng Y Q, Zhang Y, et al. The Chinese Journal of Clinical Pharmacology (卜明华, 郑咏秋, 张颖, 等. 中国临床药理学杂志), 2012, 28(1): 149

[4] Zhang G P, Zhu L J, Zhou J, et al. China Journal of Chinese Materia Medica (张广平, 朱丽君, 周娟, 等. 中国中药杂志), 2012, 37(15): 2206

[5] Yang X B, Liu J X. China Journal of Chinese Materia Medica (杨鑫宝, 刘建勋. 中国中药杂志), 2012, 37(7): 871

[6] Han Y L, Li D, Sun X P, et al. China Pharmacy (韩永龙, 李丹, 孙习鹏, 等. 中国药房), 2010, 21(3): 274

[7] Towbin H, Staehelin T, Gordon J. Proc Natl Acad, 1979, 76(9): 4350

[8] Shimada T, Yamazaki H, Mimura M, et al. J Pharmacol Exp Ther, 1994, 270: 414

[9] Bustin S A, Benes V, Nolan T, et al. J Mol Endocrinol, 2005, 34(3), 597

[10] Bustin S A. J Mol Endocrinol, 2002, 29(1): 23

[11] Bustin S A, Nolan T J. Biomol Tech, 2004, 15(3): 155

[12] Pfaffl M W. Nucleic Acids Res, 2001, 29(9): e45

[13] Rodriguez-Antona C, Jover R, Gomez-Lechon M J, et al. Biochem Biophys, 2000, 376(1): 109

[14] Morris D L, Davila J C. Biochem Pharmacol, 1996, 52(5): 781

[15] Bustin S A. J Mol Endocrinol, 2000, 25(2): 169

[16] Caron E, Rioux N, Nicolas O, et al. J Biochem Mol Toxicol, 2005, 19(6): 368

[17] Wang X, Lee W Y, Or P M, et al. Phytomedicine, 2010, 17(3/4): 203

[18] Rhodes S P, Otten J N, Hingorani G P, et al. J Pharmacol Toxicol Methods, 2011, 63(3): 223

[19] Johansson M, Tomankova J, Li S, et al. Interdiscip Toxicol, 2012, 5(3): 150

[20] Yuan R, Madani S, Wei X X, et al. Drug Metab Dispos, 2002, 30(12): 1311

[21] Donato M T, Castell J V. Clin Pharmacokinet, 2003, 42(2): 153

[22] Spaggiari D, Geiser L, Daali Y, et al. J Pharm Biomed Anal, 2014, 101: 221

[23] Kiyonami R, Schoen A, Prakash A, et al. Mol Cell Proteomics, 2011, 10(2): M110.002931

[24] Yu A M, Qu J, Felmlee M A, et al. Drug Metab Dispos, 2009, 37(1): 170

[25] Seibert C, Davidson B R, Fuller B J, et al. J Proteome Res, 2009, 8(4): 1672

[26] Fallon J K, Harbourt D E, Maleki S H, et al. Drug Metab Lett, 2008, 2(3): 210

[27] Kawakami H, Ohtsuki S, Kamiie J, et al. J Pharm Sci, 2011, 100(1): 341

[28] Ohtsuki S, Schaefer O, Kawakami H. Drug Metab Dispos, 2012, 40(1): 83

[29] Liu X, Hu L, Ge G, et al. Proteomics, 2014, 14(16): 1943

[30] Gröer C, Busch D, Patrzyk M. J Pharm Biomed Anal, 2014, 100: 393

[31] Beynon R J, Doherty M K, Pratt J M, et al. Nat Methods, 2005, 2(8): 587

[32] Achour B, Russell M R, Barber J, et al. Drug Metab Dispos, 2014, 42(4): 500

[33] Li J, Zhou L, Wang H, et al. Analyst, 2015, 140(4): 1281

[34] Ong S E, Blagoev B, Kratchmarova I, et al. Mol Cell Proteomics, 2002, 1(5): 376

[35] MacLeod A K, Fallon P G, Sharp S, et al. Mol Cell Proteomics, 2015, 14(3): 750

[36] Liu Z C, Yang F, Yu K J, et al. Chinese Journal of Chromatography (刘正才, 杨方, 余孔捷, 等. 色谱), 2012, 30(12): 1253

[37] Zhu F, Ruan L P, Ma Y, et al. Chinese Journal of Chromatography (朱峰, 阮丽萍, 马永, 等. 色谱), 2014, 32(1): 13

[38] Zhou F, Lu Y, Ficarro S B, et al. Nat Commun, 2013, 4: 2171

[39] Sato Y, Miyashita A, Iwatsubo T, et al. Drug Metab Dispos, 2012, 40(7): 1389

[40] Sato Y, Nagata M, Kawamura A, et al. Xenobiotica, 2012, 42(9): 823

[41] Alterman M A, Kornilayev B, Duzhak T, et al. Drug Metab Dispos, 2005, 33(9): 1399

[42] Al Ali A, Touboul D, Le Car J P, et al. Anal Bioanal Chem, 2014, 406(20): 4861

[43] Zhao Y, Jia W, Sun W, et al. J Proteome Res, 2010, 9(6): 3319

[44] Wang X, Qin W, Lin H, et al. Analyst, 2013, 138(18): 5309

[45] Kuzyk M A, Smith D, Yang J, et al. Mol Cell Proteomics, 2009, 8(8): 1860  "