Simultaneous determination of monosaccharide and oligosaccharide in rice wine by high performance anion exchange chromatography with integrated pulsed amperometric detection and the establishment of their fingerprints

doi:10.3724/SP.J.1123.2015.02053

Abstract

Abstract:

An analytical method for the determination of glucose, fructose, isomaltose, isomaltotriose, maltose, panose and maltotriose was developed by high performance anion exchange chromatography coupled with integrated pulsed amperometric detection. The analysis was performed on a CarboPac^TM10 column with gradient elution of NaOH-NaOAc as mobile phases. The seven sugars were well separated in less than 26 min and showed good linear correlation coefficients between 0.5 and 50 mg/L. The detection limits (LODs) were 0.1 g/L. The recoveries ranged from 76.5% to 108.4% at different spiked levels. The relative standard deviations were between 3.02% and 8.23%. The established method was applied in determining the seven sugars in different batches of Shaoxing Jiafan rice wines from different manufacturers. The standard fingerprints were established by median values using the obtained results. Similarities of the fingerprints of the samples to the standard were calculated by using angle cosine. The results showed that the similarities of wines from different manufacturers were significantly different, so the method could be used for the identification of rice wines from different manufacturers.

Key words: fingerprints, high performance anion exchange chromatography (HPAEC), integrated pulsed amperometric detection (PAD), monosaccharide, oligosaccharide, rice wines

CLC Number:

O658

HU Beizhen, DONG Wenhong, XIA Biqi, SONG Weihua, HAN Chao. Simultaneous determination of monosaccharide and oligosaccharide in rice wine by high performance anion exchange chromatography with integrated pulsed amperometric detection and the establishment of their fingerprints[J]. Chinese Journal of Chromatography, 2015, 33(6): 662-666.

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