Selective separation and determination of isoproterenol on thin layers of bismuth silicate ion-exchanger

doi:10.3724/SP.J.1123.2015.01014

Abstract

Abstract:

A simple and sensitive method for the separation and determination of isoproterenol from other doping drugs has been developed on thin layers of bismuth silicate, a synthetic inorganic ion exchanger as adsorbent in thin layer chromatography (TLC). A mixture of methanol and 0.1 mol/L formic acid (3:7, v/v) was employed as the mobile phase. The development time was 32 min. The quantitative measurement were performed with a Camag TLC Scanner-3 at wavelength (λ) of 410 nm. The isoproterenol recovery in this procedure was 98.9%. The linear correlation coefficient was greater than 0.9871 and the relative standard deviation (RSD) was less than 0.94. The limit of detection (LOD) and limit of quantification (LOQ) were 7.7×10^-7mol/L and 3.85 ×10^-6mol/L, respectively. This method has been applied in the determination of isoproterenol in dosage forms and in biological fluids.

Key words: densitometry, high performance thin layer chromatography (HPTLC), inorganic ion-exchanger

CLC Number:

O658

Vanik GHOULIPOUR, Zahra HASSANKHANI-MAJD. Selective separation and determination of isoproterenol on thin layers of bismuth silicate ion-exchanger[J]. Chinese Journal of Chromatography, 2015, 33(6): 667-671.

References

[1] Conway L, Morgan D. Drugs in Sports. London: British Medical Association, 2002

[2] Jimnez C, Ventura R, Segura J. J Chromatogr B, 2002, 767: 341

[3] Mueller R K, Grosse J, Lang R, et al. J Chromatogr B, 1995, 674: 1

[4] Sherma J, Fried B. Handbook of Thin-Layer Chromatography. New York: Marcel Dekker Inc., 1991: 407

[5] Sherma J. Anal Chem, 2010, 82: 4895

[6] Qureshi M, Varshney K G. Inorganic Ion-Exchangers in Chemical Analysis. Florida: CRC Press, 1991

[7] Husain S W, Ghoulipour V, Sepahrian H. Acta Chromatogr, 2004, 14: 102

[8] Bowers L D, Podraza J. USADA Guide to Prohibited Classes of Substances and Prohibited Methods of Doping. Colorado: Anti-Doping Agency, 2002: 7

[9] Shaw P A, Yu W A. Life Sci, 2001, 70: 301

[10] Nieto J L, Laviada I D, Guillen A, et al. Cell Signal, 1993, 5: 169

[11] Krenek P, Kmecova J, Kucerova D, et al. Eur J Heart Fail, 2009, 11(12): 140

[12] Watson J R, Lawrence R C. J Pharm Sci, 1977, 66: 560

[13] Liu Y M, Cao J T, Zheng Y L, et al. J Sep Sci, 2008, 31(13): 2463

[14] Zhou G J, Zhang G F, Chen H Y. Anal Chem Acta, 2002, 463: 257

[15] Zhang C, Huang J, Zhang Z, et al. Anal Chem Acta, 1998, 374: 105

[16] Al-Warthan A A, Al-Tamrah S A, Al-Akel A. Anal Sci, 1994, 10: 449

[17] Bonifacio Y G, Marccoline-Junior L H, Fatibello-Filho O. Anal Lett, 2004, 37(10): 2111

[18] Lupetti K O, Vieira I C, Fatibello-Filho O. Talanta, 2002, 57: 135

[19] Solieh P, Polydorou C K, Koupparis M A, et al. J Pharm Biomed Anal, 2000, 22: 781

[20] Nevado J J B, Gallego J M L, Laguna P B. Anal Chem Acta, 1995, 300: 293

[21] Ensafi A A, Khoddami E, Karimi-Maleh H. Int J Electrochem Sci, 2011, 6: 2596

[22] Kutluay A, Aslanoglu M. Acta Chim Slov, 2010, 57: 157

[23] Elord J L, Schmit J L, Morley J A. J Chromatogr A, 1996, 723: 235

[24] Hassankhani-Majd Z, Ghoulipour V, Husain S W. Acta Chromatogr, 2006, 16: 173

[25] Ahuja S, Scypinski S. Handbook of Modern Pharmaceutical Analysis. Burlington: Academic Press, 2011: 430

[26] Ghoulipour V, Husain S W. Anal Sci, 2000, 16: 1079

[27] ICH Guidelines Q2B, Validation of Analytical Procedures: Methodology (CPMP/ICH/281/95). Geneva, 1996

[28] Papp E, Farkas A, Otta K H, et al. J Planar Chromatogr, 2000, 13: 328

[29] Ferenczi-Fodor K, Vegh Z, Nagy-Turak A, et al. J AOAC Int, 2001, 84: 1265

[30] Dallas F A A, Read H, Ruane R J, et al. Recent Advances in Thin-Layer Chromatography. New York: Springer, 1988: 11

[31] Ferenczi-Fodor K, Renger B, Vegh Z. J Planar Chromatogr, 2010, 23: 173

[32] Ramirez A, Gutierrez R, Diaz G, et al. J Chromatogr B, 2003, 784: 315

[33] Mazlom-Ardakani M, Sabaghian F, Khoshroo A, et al. Chin J Catal, 2014, 35: 565

[34] Lupetti K O, Vieira I C, Fatibello-Filho O. Talanta, 2002, 57: 135

[35] Rezaei B, Ensafi A A, Haghighatnia F. Anal Methods, 2012, 4: 1753

[36] Rocha F R P, Nobrega J A, Fatibello D. Green Chem, 2001, 3: 216