Chinese Journal of Chromatography ›› 2015, Vol. 33 ›› Issue (12): 1226-1233.DOI: 10.3724/SP.J.1123.2015.09010

• Reviews • Previous Articles     Next Articles

Recent advances in preparative electromigration techniques for proteins

HAO Feiran, FU Bin, ZHANG Yangjun, QIAN Xiaohong   

  1. State Key Laboratory of Proteomics, Beijing Proteome Research Center, Beijing Institute of Radiation Medicine, Beijing 102206, China
  • Received:2015-09-14 Online:2015-12-08 Published:2012-06-21

Abstract:

Preparative electromigration techniques are a category of separation and preparation techniques based on differential electromigration principle. These techniques are widely applied in biomacromolecule and proteome researches. Related techniques mainly include preparative electrophoresis, preparative electrochromatography, preparative isoelectric focusing and free flow electrophoresis and so on. We review each kind of the preparative electromigration techniques on its apparatus designs, applications, advantages and disadvantages in detail. Microscale preparative electrophoresis is a hot spot in recent years. Its high resolution, high recovery and high efficiency make it a more prominent role in the biological sample analysis. In this review, we focus on some advances in microscale preparative electrophoresis and also give an outlook on preparative electromigration techniques in the future.

Key words: free flow electrophoresis, microscale preparative electrophoresis, preparative electrochromatography, preparative electromigration technique, preparative electrophoresis, preparative isoelectric focusing, proteins, review

CLC Number: