Rapid screening and confirmation of non-target pigment in Chinese softshell turtle by liquid chromatography coupled to time of flight mass spectrometry

doi:10.3724/SP.J.1123.2015.08008

Abstract

Abstract:

A method of non-target pigment screening in Chinese softshell turtle has been established by using liquid chromatography coupled to time of flight mass spectrometry (LC-Q-TOF MS). After being purified by a simple acetonitrile extraction work, the non-target pigment in 20 Chinese softshell turtle samples was detected by liquid chromatography-photodiode array detection (LC-DAD). The S7 sample, which has a strong spectral response, was chosen to extract the mass spectrometry information of the non-target pigment on different gradient elution conditions. In order to get the characteristic molecular mass ion (564.39773 Da and 564.39561 Da) of the non-target pigment, qualitative spectral full scan with negative sample was used. The molecular formula generation data and the literature information prompted speculation that the non-target pigment was canthaxanthin with the formula of C₄₀H₅₂O₂. By comparing the canthaxanthin standard material MS/MS information, the result was confirmed accurate. A strategy of LC-Q-TOF MS method for the qualitative analysis of unknown compounds is discussed, and the results indicated that the described method can be effectively applied to qualitative analysis for non-target pigment in Chinese softshell turtle.

Key words: Chinese softshell turtle, liquid chromatography-quadrupole time of fight mass spectrometry (LC-Q-TOF MS), non-target compound identification, pigment

CLC Number:

O658

LI Shiyan, WANG Yang, ZHOU Fan, ZHENG Chongying, ZHANG Haiqi, HE Zhongyang, HE Xin. Rapid screening and confirmation of non-target pigment in Chinese softshell turtle by liquid chromatography coupled to time of flight mass spectrometry[J]. Chinese Journal of Chromatography, 2015, 33(12): 1251-1256.

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