Relative multicomponent quantitative and identification method of bugloss based on the fingerprint

doi:10.3724/SP.J.1123.2016.05011

Abstract

Abstract:

Chromatographic fingerprint plays a key role in the identification of habitat for Chinese herbs or Chinese patent medicines. However, peaks' intensities and positions can be affected by the extraction and separation conditions of target, and it is very significant to develop a more precise fingerprint. The certain component (rutin) as the internal standard was used to confirm the relative contents of other components, which was applied in chemical pattern recognition. To verify the idea, according to the peak of rutin as reference, the relative contents of the other defined fingerprint peaks were obtained. A multicomponent quantitative and identification method was established by the fingerprint of bugloss. The obtained fingerprints were used for the chemical pattern recognition, including hierarchical cluster analysis and the principal component analysis, and the bugloss collected from different places could be distinguished effectively. A relative multicomponent quantitative and identification method for bugloss was established. The established method is economic, feasible, and significant for quality control of other herbs and patent medicines.

Key words: bugloss, chemical pattern recognition, fingerprint, quantitative method

CLC Number:

O658

CHU Ganghui, MUHETAER·Tu'erhong, YIN Xuebo, MUNIRA·Abudukeremu. Relative multicomponent quantitative and identification method of bugloss based on the fingerprint[J]. Chinese Journal of Chromatography, 2016, 34(9): 901-905.

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