Advances of Caenorhabditis elegans research on microfluidic device

doi:10.3724/SP.J.1123.2016.07017

Abstract

Abstract:

The nematode Caenorhabditis elegans (C. elegans) has been widely used as a multicellular organism for biological research due to its simplicity, short life cycle, and relevance to human genetics and biology. In the past decade, microfluidics technology was emerging as a promising platform for the study of C. elegans both in continuous flow-based or droplet-based format. Worm long-term culture, immobilization, sorting, stimuli exposure and individual worm encapsulation have been realized on microfluidic platform. By providing unique capabilities with precise manipulations and controlled microenvironment, this platform has been used for the study of neurobiology, behaviors, aging and development, and drug screening in C. elegans. In this review, we highlight the recent advances of microfluidic technology in worm manipulations and its biomedical applications. The prospects and challenges of microfluidic technology for future studies are discussed as well.

Key words: Caenorhabditis elegans (C. elegans), droplets, microfluidics, review

CLC Number:

O658

YIN Fangchao, WEN Hui, ZHU Guoli, QIN Jianhua. Advances of Caenorhabditis elegans research on microfluidic device[J]. Chinese Journal of Chromatography, 2016, 34(11): 1031-1042.

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