Rapid screening of 27 pesticide residues in chili powder by ultra performance liquid chromatography coupled with quadrupole-time of flight mass spectrometry

doi:10.3724/SP.J.1123.2016.07007

Abstract

Abstract:

An analytical method was established for the simultaneous determination of 27 pesticide residues in chili powder by ultra performance liquid chromatography coupled with quadrupole-time of flight mass spectrometry (UPLC-Q-TOF/MS). The samples were extracted with acetonitrile, and cleaned-up by solid phase extraction (SPE) with Carb/NH₂ cartridges, and eluted with acetonitrile-ethyl acetate (3: 1, v/v). The eluent was determined by UPLC-Q-TOF/MS with electrospray ionization in positive mode. The confirmatory analysis was carried out by comparing the retention times, accurate masses, isotope abundance and isotope spacing of all the compounds and fragment ions with Targeted MS/MS. The correlation coefficients (r) were greater than 0.997 in the linear ranges of the 27 pesticides. The limits of quantification (LOQs) of the 27 pesticides were 2.5-5.0 μg/kg. At the three spiked levels, the recoveries were between 72.3% and 118.9%, with the relative standard deviations (RSDs, n=6) ranging from 0.21% to 12.7%. The method is efficient, sensitive and accurate, and can meet the requirement of the determination of the 27 pesticides in chili powder.

Key words: chili powder, pesticide residues, screening, ultra performance liquid chromatography-quadrupole-time of flight mass spectrometry (UPLC-Q-TOF/MS)

CLC Number:

O658

LIU Yongqiang, LI Kai, XU Wenjuan, GUO Liqiang, SUN Jun. Rapid screening of 27 pesticide residues in chili powder by ultra performance liquid chromatography coupled with quadrupole-time of flight mass spectrometry[J]. Chinese Journal of Chromatography, 2016, 34(11): 1055-1062.

References

[1] Li F P. Modern Food, 2016(4):61 黎凤平. 现代食品, 2016(4):61
[2] Liu B S, Lü Y K, Guo C H, et al. Chemical Analysis and Meterage, 2011, 20(3):50 刘宝圣, 吕运开, 郭春海, 等. 化学分析计量, 2011, 20(3):50
[3] Li H C, Zhang Y P, Hu D Y, et al. Chinese Journal of Pesticide Science, 2013, 15(1):117 李海畅, 张钰萍, 胡德禹, 等. 农药学学报, 2013, 15(1):117
[4] Zhang Q, Chen W S, Hong L, et al. Food Science, 2014, 35(8):295 张权, 陈文生, 洪亮, 等. 食品科学, 2014, 35(8):295
[5] Liu H S, Feng G, Liu P, et al. Journal of Food Safety and Quality, 2015, 6(4):1498 刘会双, 冯刚, 刘萍, 等. 食品安全质量检测学报, 2015, 6(4):1498
[6] Sun J F, Zhao J H, Xiong G, et al. Journal of Instrumental Analysis, 2011, 30(10):1094 苏建峰, 赵建晖, 熊刚, 等. 分析测试学报, 2011, 30(10):1094
[7] Li X Y, Zhang H Y, Fang C L, et al. Chemistry, 2014, 77(2):123 李晓颍, 张红医, 范春林, 等. 化学通报, 2014, 77(2):123
[8] Li K, Liu Y Q, Zhang J L, et al. Chinese Journal of Analysis Laboratory, 2015, 34(2):236 李凯, 刘永强, 张金玲, 等. 分析试验室, 2015, 34(2):236
[9] Jia W, Huang J R, Ling Y, et al. Journal of Instrumental Analysis, 2013, 32(1):9 贾玮, 黄峻榕, 凌云, 等. 分析测试学报, 2013, 32(1):9
[10] Zhang A Z, Wang Q L, Cao L L, et al. Chinese Journal of Chromatography, 2016, 34(2):158 张爱芝, 王全林, 曹丽丽, 等. 色谱, 2016, 34(2):158
[11] Yu L, Song W, Lü Y N, et al. Chinese Journal of Chromatography, 2015, 33(6):597 余璐, 宋伟, 吕亚宁, 等. 色谱, 2015, 33(6):597
[12] Zhao Z Y, Shi Z H, Kang J, et al. Chinese Journal of Chromatography, 2013, 31(4):372 赵志远, 石志红, 康健, 等. 色谱, 2013, 31(4):372
[13] Peng X, Zhao Z Y, Kang J, et al. Chinese Journal of Analysis Laboratory, 2014, 33(3):282 彭兴, 赵志远, 康健, 等. 分析试验室, 2014, 33(3):282
[14] Zhu W Y, Zhang X, Yang J, et al. Chinese Journal of Chromatography, 2015, 33(9):1002 朱万燕, 张欣, 杨娟, 等. 色谱, 2015, 33(9):1002
[15] Li X J, Peng T, Li C J. Journal of Instrumental Analysis, 2012, 31(5):628 李晓娟, 彭涛, 李重九. 分析测试学报, 2012, 31(5):628
[16] Pan Y X, Dong J, Sun J, et al. Physical Testing and Chemical Analysis Part B:Chemical Analysis, 2009, 45(8):991 潘玉香, 董静, 孙军, 等. 理化检验-化学分册, 2009, 45(8):991
[17] Huang Y S, Luo X, Xiong H L, et al. Chinese Journal of Analysis Laboratory, 2013, 32(10):72 黄优生, 罗香, 熊华亮, 等. 分析试验室, 2013, 32(10):72
[18] Wang L Q, He L M, Zeng Z L, et al. Journal of Chinese Mass Spectrometry Society, 2011, 32(6):321 王立琦, 贺利民, 曾振灵, 等. 质谱学报, 2011, 32(6):321