Analysis of trace estrogens in toners by monolithic column online solid phase microextraction coupled with high performance liquid chromatography

doi:10.3724/SP.J.1123.2016.08012

Abstract

Abstract:

A Poly(glycidyl methacrylate-co-ethyleneglycol dimethacrylate) (Poly(GMA-co-EDMA)) monolithic column was prepared in a capillary by in-situ polymerization. Glycidyl methacrylate (GMA) was chosen to be the monomer and ethyleneglycol dimethacrylate (EDMA) was utilized as crosslinking agent. The binary porogenic agents of methanol and n-hexane can develop the permeability of monolithic column and they were suitable to construct the online analysis apparatus. A series of experiments were carried out, and they demonstrated the monolithic column had good permeability with low column pressure (1.5×10⁶ Pa at the washing flow rate of 0.5 mL/min). The enrichment factors of the prepared monolithic column for estradiol, ethynyl estradiol, estrone and diethylstilbestrol were 86, 116, 77 and 86, respectively. It was proved that the monolithic column was suitable for the online microextraction system by using the interface unit. A method was established for the analysis of trace estradiol, ethynyl estradiol, estrone and diethylstilbestrol in toners by monolithic column online solid phase microextraction coupled with high performance liquid chromatography. The limits of detection (S/N=3) were 0.05-0.20 μg/L. The recoveries in toner samples varied from 69.3% to 111.3%, and relative standard deviations were less than 5.0%. The method is efficient, sensitive, accurate, reliable and applicable to analyze trace estrogens in toners.

Key words: estrogens, high performance liquid chromatography (HPLC), online solid phase microextraction, poly(glycidyl methacrylate-co-ethyleneglycol dimethacrylate) (Poly(GMA-co-EDMA)) monolithic column, toners

CLC Number:

O658

SONG Wenwen, LUO Xialin, LI Gongke, HU Yufei. Analysis of trace estrogens in toners by monolithic column online solid phase microextraction coupled with high performance liquid chromatography[J]. Chinese Journal of Chromatography, 2017, 35(1): 20-26.

References

[1] Ocana-González J A, Villar-Navarro M, Ramos-Payán M, et al. Anal Chim Acta, 2015, 858: 1
[2] Jain A, Soni S, Verma K K. J Liq Chromatogr R T, 2015, 38(1): 82
[3] Wang D H, Dong Z Q, Wang Y L, et al. Chinese Journal of Pest Control, 2009, 25(5): 350 王大虎, 董智强, 王玉林, 等. 医学动物防制, 2009, 25(5): 350
[4] Mei M, Yu J, Huang X J, et al. J Chromatogr A, 2015, 1385: 12
[5] Studzińska S, Buszewski B. J Chromatogr B, 2013, 927: 158
[6] Xiao X H, Yin Y, Hu Y L, et al. Chinese Journal of Chromatography, 2007, 25(2): 234 肖小华, 尹怡, 胡玉玲, 等. 色谱, 2007, 25(2): 234
[7] Castanedo-Tardan M P, Zug K A. Dermatol Clin, 2009, 27(3): 265
[8] Darbre P D. Best Pract Res Cl En, 2006, 20(1): 121
[9] Lesellier E, West C. J Chromatogr A, 2015, 1382: 2
[10] Pan C J, Wang W F, Chen X G. J Chromatogr A, 2016, 1427: 125
[11] Chen L F, Ou J J, Liu Z S, et al. J Chromatogr A, 2015, 1394: 103
[12] Zhang Z G, Liu J G, Li J S, et al. Fine and Specialty Chemicals, 2015, 23(1): 9 张志刚, 刘金刚, 李婧实, 等. 精细与专用化学品, 2015, 23(1): 9
[13] Zhou S Y, Chen J Y, Tan J J, et al. Chinese Journal of Chromatography, 2015, 33(12): 1307 周孙英, 陈继涢, 谭静静, 等. 色谱, 2015, 33(12): 1307
[14] Niu X Y, Ding S Y, Wang W F, et al. J Chromatogr A, 2016, 1436: 109
[15] Brabcová I, Satínsky D, Solich P. Anal Methods, 2013, 5(21): 6153
[16] Li J R, Ma Q, Meng X S, et al. Journal of Instrumental Analysis, 2015, 34(1): 43 李晶瑞, 马强, 孟宪双, 等. 分析测试学报, 2015, 34(1): 43
[17] Yan W, Li Y, Zhao L X, et al. J Chromatogr A, 2009, 1216(44): 7539
[18] Wang P J, Xiao Y, Liu W J, et al. Food Chem, 2015, 172: 385
[19] Yang S S, Yang Y N, Li X L, et al. Chinese Journal of Analytical Chemistry, 2016, 44(3): 396 杨姗姗, 杨亚楠, 李雪霖, 等. 分析化学, 2016, 44(3): 396
[20] Zhang J M, Lin W S, Li X N, et al. J Sep Sci, 2012, 35(5/6): 721
[21] Wu R A, Zou H F, Ye M L, et al. Anal Chem, 2001, 73: 4918
[22] Liu Y, Wang M M, Ai L F, et al. J Sep Sci, 2014, 37(13): 1648
[23] Wang J, Zhou Z R, Lin S B. Journal of Hygiene Research, 2010, 39(3): 383 王君, 周志荣, 林少彬. 卫生研究, 2010, 39(3): 383
[24] Liu Y, Zhang X L, Ouyang Y, et al. J Mass Spectrom, 2011, 46(8): 794