Rapid determination of 95 herbicide residues in tea by modified QuEChERS coupled with ultra-high performance liquid chromatography-quadrupole/electrostatic field orbitrap high resolution mass spectrometry

doi:10.3724/SP.J.1123.2018.08027

Abstract

Abstract:

A qualitative and quantitative method was established based on modified QuEChERS for the simultaneous determination of 95 herbicide residues in tea using ultra-high performance liquid chromatography-quadrupole/electrostatic field orbitrap high resolution mass spectrometry (UHPLC-Q-Orbitrap HRMS). The effects of multi-walled carbon nanotubes (MWCNTs), graphitized carbon black (GCB), primary secondary amine (PSA) and toluene on the precondition step were evaluated in terms of matrix-spiked recovery and pigment clean-up effect. Finally, the modified QuEChERS method was applied, which involved sample extraction with an acetonitrile-toluene (9:1, v/v) mixture containing 1% (v/v) acetic acid, followed by cleaning with 12.5 mg GCB, 12.5 mg MWCNTs and 150 mg PSA. The sample extract was separated on a Hypersil Gold C18 column and analyzed in full scan/data-dependent MS²(Full MS/dd-MS²) mode. The target herbicides were quantified by using the matrix-matched standard calibration. The three-level spiked recoveries were between 63.3% and 129.1% with the precision of 0.7%-15.2%. This method is easy, sensitive and rapid and can be applicable to the determination of trace herbicide residues in tea and other plant-derived complex matrix samples.

Key words: herbicides, modified QuEChERS, multi-walled carbon nanotubes (MWCNTs), quadrupole/electrostatic field orbitrap high resolution mass spectrometry (Q-Orbitrap HRMS), tea, ultra-high performance liquid chromatography (UHPLC)

CLC Number:

O658

ZHANG Rong, LIU Xin, PENG Yuan, LIU Weihua, ZHANG Licheng, DAI Ying, LI Shasha, GAO Zhixian. Rapid determination of 95 herbicide residues in tea by modified QuEChERS coupled with ultra-high performance liquid chromatography-quadrupole/electrostatic field orbitrap high resolution mass spectrometry[J]. Chinese Journal of Chromatography, 2018, 36(12): 1228-1237.

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